北京地区一株葡萄酸腐病相关酵母菌的分离鉴定

Isolation and identification of a yeast strain associated with grape sour rot in Beijing

近年来葡萄酸腐病在中国葡萄主要产区发生普遍而严重,已成为葡萄生产上的重要病害之一,但目前该病害的病原不明确且存在争议。采用组织分离法从北京地区采集的葡萄酸腐病病果上分离纯化出一株酵母菌sf-19,采用形态学特征观察、Biolog微生物自动分析系统和26SrDNA D1/D2区序列分析,将菌株sf-19鉴定为葡萄汁有孢汉逊酵母(Hanseniaspora uvarum)。致病性测定发现,酵母菌sf-19菌株可引起葡萄果实腐烂,且从接种腐烂的果实中又分离出葡萄汁有孢汉逊酵母。结果表明:葡萄汁有孢汉逊酵母是引起中国葡萄酸腐病的相关酵母菌之一。

Sour rot is one of the most important grape diseases causing great production loss and lower quality of grape and occurs in main grape-producing regions in China.However,etiology of the disease is ambiguous at worldwide.A yeast strain sf-19 was isolated by the tissue culture technique from diseased grape fruits collected in Beijing field.Based on the analysis results of Biolog microbial identification system,26 SrDNA D1/D2 domain sequence analysis,morphological and physiological characteristics,sf-19 was identified as Hanseniaspora uvarum.In addition,the pathogenicity of strain sf-19 was tested and it showed that the strain caused fruit rot on grape.The original yeast was re-isolated from inoculated and diseased fruits.The studies showed that H.uvarum is one of pathogenic yeasts associate with grape sour rot in China.