高粱SbNAC1基因克隆及初步分析

Cloning and sequence analysis of SbNAC1 in sorghum(Sorghum bicolor L.)

【目的】在植物抗逆信号转导途径中,NAC转录因子处于承上启下的关键位置,通过调控靶基因的表达发挥抗逆功能。本研究克隆高粱SbNAC1基因全长cDNA,并对其进行序列比对、进化分析,为深入研究其在作物抗逆反应中的生物学功能奠定基础。【方法】RT-PCR扩增SbNAC1全长cDNA,并对其进行基因序列比对和系统进化分析等生物信息学分析。【结果】SbNAC1cDNA全长966bp,编码321个氨基酸。氨基酸序列比对结果表明高粱SbNAC1与水稻SNAC1同源性达到83.69%。

Transcription factors play vital roles in plant response to abiotic stresses.In signaling transduction pathways,NAC transcription factors regulate the expression of stress responsive genes by interacting with relevant cis-elements in gene promoters and play essential roles in stress tolerance.The full-length cDNA of SbNAC1 was isolated from sorghum using RT-PCR method.The full-length cDNA of SbNAC1 is 966bp,which encoded 321 amino acids.A series bioinformatics analyses including the multiple sequence alignment,phylogenetic analysis of SbNAC1 were performed with various software.Multiple sequence alignment revealed that the deduced SbNAC1 protein exhibited the highest(83.69%)sequence identity to rice SNAC1.