桔梗(Platycodon grandiflorum(Jacq.)A.DC.)的组织培养和植株再生

Tissue culture and plant regeneration of Platycodongrandiflorum(Jacq.)A.DC.

为了建立桔梗(Platycodon grandiflorum(Jacq.)A.DC.)的组织培养快速繁殖体系,以桔梗的幼茎和幼叶为外植体,进行愈伤组织及再生芽的诱导。研究结果表明,幼茎较容易脱分化与再分化,发现3种激素组合中6-BA、2,4-D和NAA,MS+6-BA 0.5mg/L+NAA 1.0mg/L为最佳的愈伤组织诱导及芽再生培养基;在3种生长素组合中,IAA、IBA和NAA分别诱导,1/2 MS+NAA 0.1mg/L为最佳,其生根率90%以上。

Platycodon grandiflorum(Jacq.)A.DC.,is a double duty plant as traditional herb medicine and special vegetable in China.In order to establish the tissue culture and rapid propagation system,the young stem segments and young leaves of P.grandiflorum(Jacq.)A.DC.were used as explants to induce the callus and regeneration buds.The results showed that young stem segments were easier than young leaves in dedifferentiation and redifferentiation.Among different combinations of 6-BA,NAA,and 2,4-D,the MS medium containing 0.5mg/L 6-BA,and 1.0mg/L NAA was optimal to induce the callus and regeneration buds.Among three auxins(IAA,IBA,and NAA),1/2MS medium containing 0.1mg/L NAA was the optimum rooting medium,and the rooting rate exceeded 90%.