TRV-GFP载体在麦秆菊上的应用

Application of TRV-GFP vector in strawflower plants

病毒介导基因沉默(Virus-induced gene silencing,VIGS)对于研究基因功能是一种快速有效的反向遗传学工具。然而,高效的VIGS的技术却只在很少的一些植物中得到应用。为了拓展VIGS技术的应用,探明烟草脆裂病毒(Tobacco Rattle Virus,TRV)是否可以侵染麦秆菊(Helichrysum bracteatum Andr.)。利用以TRV-GFP为载体的VIGS方法成功侵染麦秆菊。由于TRV病毒载体携带有绿色荧光蛋白(Green Fluorescent Protein,GFP)的编码序列,所以利用荧光显微镜和手提式紫外灯可以迅速检测到TRV-GFP标记蛋白,利用GFP荧光的检测可以筛选侵染植株。总之,本研究证实以TRV-GFP为载体的VIGS方法为麦秆菊基因功能组的研究提供一个崭新的、潜在的、有价值的工具。

Virus-induced gene silencing(VIGS)is an attractive reverse-genetics tool for studies of plant gene function;however,efficient VIGS has only been accomplished in a few plant species.In order to extend the application of VIGS,we examined whether a VIGS vector based on the tobacco rattle virus(TRV)would result in phenotypic changes in strawflower(Helichrysum bracteatum Andr.).In this study,we successfully infiltrated strawflower using TRV-GFP vector.The spread of the virus harboring the TRV vector containing the coding sequence of the green fluorescent protein(GFP)marker protein(TRV-GFP)virus was readily monitored using fluorescent microscopy and a hand-held UV lamp,such that the infiltrated plants could be screened by detecting the GFP fluorescence.Taken together,the results indicate that VIGS using the TRV/TRV-GFP vector represents a new potentially valuable tool for strawflower functional genomic studies.