5种苹果属植物GAI基因的克隆及生物信息学分析

Cloning and bioinformatic analysis of GAI in the five Malus species

【目的】研究DELLA家族的成员GAI在调控植物株型形成方面的生物学功能。【方法】从5种苹果属植物(‘国光’、‘武乡海棠’、‘SH6’、‘比利时垂枝’、‘比利时直立’)中分离得到GAI基因,应用多种生物学工具分析其序列特征。【结果】序列分析表明,GAI的CDS序列长度为1 875bp,编码624个氨基酸;GAI的基因组序列长度与其对应CDS序列长度相同;GAI蛋白具有亲水性,主要定位于细胞质;5种苹果属植物的GAI序列相似性极高,都具有DELLA家族和GRAS家族特有的典型结构。【结论】试验结果暗示这5种苹果属GAI基因可能在苹果株型形成方面发挥重要功能。

【Objective】Our research is aimed to study the functions of GAIs,members of DELLA protein family,in regulating the plant architecture.【Methods】In this study,GAIs,were isolated from the five Malus sp.cultivars(‘Ralls Janet',‘Honanensis Rehd',‘SH6',‘Belgium weeping',‘Belgium upright'),and their sequence analyses were performed by lots of bioinformatic tools.【Results】The results indicated that the full length CDSs of GAIs of these five Malus cultivars were 1 875 bp,encoding 624 amino acids.As to each gene,genomic sequence of GAI was the same as its CDS sequence.GAI protein was hydrophilic and mainly located in the cytoplasm.Multiple sequence alignment indicated that GAI proteins of the five Malus sp.cultivars presented high sequence homology,especially at DELLA and GRAS Super-family typical domains.【Conclusion】The study showed that GAI might play important regulatory roles in the formation of Malus tree architecture.