低温胁迫对甜瓜砧木幼苗生理生化指标的影响

Effect of low temperature stress on physiological and biochemical index of melon rootstock seedlings

【目的】研究14种不同甜瓜砧木在低温胁迫下的抗寒性,比较不同甜瓜砧木的抗寒性强弱,为甜瓜嫁接栽培及砧木耐冷性筛选提供依据。【方法】本试验经0℃低温处理,对14种不同甜瓜砧木的丙二醛含量、电导率和根系活力进行测定,其结果反映低温胁迫对甜瓜砧木细胞膜透性、根系活力等特性的影响,其中丙二醛含量采用分光光度法测定;电导率采用电导仪测定;根系活力采用氯化三苯基四氮唑法(TTC)测定。【结果】通过低温胁迫,甜瓜砧木的膜透性增大,MDA含量增加,根系活力减弱。【结论】综合分析比较后证明甜瓜砧木京欣砧8(代号A13)的抗寒性最好。

【Objective】To provide the basis for better cultivation and rootstock grafted melon cold screening,the cold resistance for 14 different melon rootstocks under low temperature stress were analyzed and compared.【Methods】The MDA content,TTC deoxidizing intensity and electrolytic leakage of 14 different melon rootstocks objected 0℃low temperature were detected,in order to determined effects of low temperature stress on cell membrane permeability and root activity.【Results】The results showed that low temperature stress caused electrolyte leakage increased,MDA content increased,TTC reductive intensity decreased,the root activity weakened.【Conclusion】Comprehensive analysis indicated that melon rootstocks Jingxin anvil 8(A13)was appeared best in cold hardiness.