四季抗病毒合剂制备中连翘酯苷A和连翘酯苷B转移率的研究

Study on the transfer rate of forsythiaside A and forsythiaside B in the preparation of Sijikangbingdu Mixture

目的建立连翘与四季抗病毒合剂中连翘酯苷A和连翘酯苷B的含量测定方法,阐明其在连翘药材至四季抗病毒合剂制剂间的转移率。方法采用超快速液相色谱(UFLC)法,选取3批连翘药材,每批对应5批制剂。结果 1 3批连翘药材中连翘酯苷A的含量分别为12.57,20.26和18.64mg·g^(-1),3批连翘药材中连翘酯苷B的含量依次分别为2.22,3.76和3.07mg·g^(-1);2每批连翘药材对应5批制剂中连翘酯苷A的平均含量分别为1 129.40,1 828.30和1 666.30μg·mL^(-1),每批连翘药材对应5批制剂中连翘酯苷B的平均含量依次分别为224.80,382.60和315.70μg·mL^(-1);3 15批制剂中连翘酯苷A和连翘酯苷B的转移率分别为74.86%和84.95%,RSD分别为0.88%和1.43%。结论该含量测定方法具有简便、稳定、可靠、准确等优点,可作为连翘与四季抗病毒合剂中连翘酯苷A和连翘酯苷B的定量方法;连翘酯苷A和连翘酯苷B的转移率高,表明制剂制备工艺有利于2种有效成分的溶出,为保证制剂疗效提供了理论依据。

Objective To establish a UFLC method for determing the contents of forsythiaside A and forsythiaside B in Forsythiae fructus and Sijikangbingdu Mixture,and clarify their transfer rate in the preparation.Methods UFLC analysis was adopted,then three batches of Forsythiae fructus were selected,each batch of which included 5batches of Sijikangbingdu Mixture.Results The contents of forsythiaside A in 3batches of Forsythiae fructus were 12.57,20.26 and 18.64mg·g^-1,respectively.And the contents of forsythiaside B in three batches of Forsythiae fructus were 2.22,3.76 and 3.07mg·g-1,respectively.The average contents of of forsythiaside A in 5batches of Sijikangbingdu Mixture were 1 129.40,1 828.30 and 1 666.30μg·mL^-1,respectively.And the average contents of forsythiaside B in 5batches of Sijikangbingdu Mixture were 224.80,382.60 and 315.70μg·mL^-1,respectively.The transfer rates of 15 batches of Sijikangbingdu Mixture in the preparation were 74.86%,and 84.95%with RSDs0.88%,and 1.43% respectively.Conclusion The method is simple,stable,reliable,and accurate,and can be used as a quantitative method of forsythiaside A,forsythiaside B in Sijikangbingdu Mixture and Forsythiae fructus.The transfer rates of forsythiaside A,forsythiaside B were high in the preparation,so which provides a theoretical basis for the preparation of curative effect.