神经生长因子mRNA在鹅等级前卵泡中的表达

Expression of Nerve Growth Factor mRNA in Pre-hierarchical Follicles of Geese

采用相对荧光定量PCR(RT-PCR)相对标准曲线方法,对籽鹅和卡洛斯鹅不同产蛋时期初级卵泡、小白卵泡、中白卵泡和大白卵泡中神经生长因子(NGF)mRNA的表达量进行研究,同时检测血液中促卵泡素(FSH)的含量,并与NGFmRNA表达量进行相关性分析。结果表明:产蛋高峰期,籽鹅初级卵泡中NGF mRNA表达量极显著高于其他卵泡(P<0.01),卡洛斯鹅初级卵泡中NGF mRNA表达量显著高于中白卵泡(P<0.05),其余卵泡间差异不显著。产蛋末期,籽鹅初级卵泡中NGF mRNA表达量极显著高于中白卵泡和小白卵泡(P<0.01),大白卵泡NGF mRNA表达量极显著高于中白卵泡(P<0.01),卡洛斯鹅初级卵泡NGF mRNA表达量极显著高于中白卵泡和小白卵泡(P<0.01),大白卵泡极显著高于其他卵泡(P<0.01)。2种鹅产蛋高峰期血液中FSH表达水平高于产蛋末期(P<0.05);籽鹅和卡洛斯鹅的小白卵泡NGF mRNA表达量与血液中FSH的表达水平呈显著正相关(P<0.05),在大白卵泡中两者呈负相关,其他卵泡呈正相关。

In this study,pre-hierarchical follicles of Zi geese and Carlos geese were used as materials. By RT-PCR technique,expression of NGF in pre-hierarchical follicles of different periods was quantitatively studied,change of follicle stimulating hormone（ FSH） in two kinds of geese blood was tested,and relationship between content of FSH and expression of NGF was explored. The results were as follows： relative expression amount of NGF in primary follicles of Zi geese is very significantly higher than that of other models except that of the largest white follicles,which is in peek period（ P〈0. 01）. Expression amount of NGF in pre-hierarchical follicles of Carlos geese is higher than that of middle white follicles（ P〈0. 05）. For Zi geese,during the last stage of laying,expression amount of NGF in pre-hierarchical follicles is very significantly higher than that of middle white follicles（ P〈0. 01）. For Carlos geese,expression amount of NGF in large white follicles is very significantly higher than that of other models（ P〈0. 01）. Expression amount of NGF in primary follicles of Zi geese peek period,the last stage of laying and Carlos geese peek period is very significantly higher than that of the last stage of Carlos geese（ P〈0. 01）. Expression amount of NGF in small white follicles in Zi geese and Carlos geese show significantly positive correlation with expression amount of FSH in serum（ P〈0. 05）. It shows a negative correlation with expression amount of FSH.And it shows a positive correlation with expression amount of FSH.