芦荟大黄素联用顺铂对MDA-MB231乳腺癌细胞Caspase-3和端粒酶表达的影响

Effects of Aloe Emodin Alone and in Combination with Cisplatin on Expression of Caspase-3 and Telomerase in Breast Cancer Cell Line MDA-MB-231

目的探讨芦荟大黄素(AE)单用或联用顺铂(CP)对乳腺癌MDA-MB-231细胞Caspase-3和端粒酶表达的影响。方法 AE单用和联用CP处理细胞后,用分光光度法检测Caspase-3酶活变化;荧光定量PCR和Western blot检测Caspase-3、h TERT和c-myc mRNA及蛋白的表达水平。结果 AE单用,Caspase-3蛋白表达量和酶活增强,Caspase-3基因表达增强,而h TERT和c-myc基因和蛋白表达量均受抑制。AE与CP联用则强化了这种趋势。结论芦荟大黄素抑制MDA-MB231乳腺癌细胞的增殖与凋亡酶的激活和端粒酶的抑制有关。

Objective To investigate the caspase- 3 and telomerase expression of breast cancer cell line MDA- MB- 231 when treated by aloe emodin（ AE） alone and in combination with cisplatin（ AE- CP）. Methods Cells were treated by AE and AE-CP. The enzyme activities of caspase- 3 were detected by spectrophotometer. The mRNA and protein levels of Caspase 3,h TERT and c- Myc were determined by real- time PCR and Western blot. Results After AE treated,the mRNA and protein levels,and the activities of caspase- 3 increased,whereas the mRNA and protein levels of h TERT and c- Myc decreased. The effects were accentuated when AE was combined with CP. Conclusion Aloe emodin activated the expression of caspase- 3 but inhibited that of telomerase.