温针灸对膝骨关节炎大鼠关节软骨及形态的影响

Effects of warm needling moxibustion on knee cartilage and morphology in rats with knee osteoarthritis

目的:观察温针灸对膝骨关节炎(knee osteoarthritis,KOA)大鼠体质量、关节软骨及关节形态的影响。方法:将40只SD大鼠随机分为正常组、模型组、药物组和温针灸组,每组10只。除正常组,其他3组采用木瓜蛋白酶注射造模法制造膝骨关节炎模型。造模后模型组不予处理,温针灸组于双侧"膝前"温针灸治疗,药物组给予美洛昔康片灌胃,正常组灌服与药物组等量的0.9%NaCl溶液,并做与温针灸组相同的固定。均每天1次,连续20d。观察治疗后各组大鼠体质量变化、关节软骨积分和关节形态变化情况。结果:体质量增长方面,药物组和温针灸组快于模型组、慢于正常组(均P<0.05),药物组与温针灸组差异无统计学意义(P>0.05);软骨积分方面,温针灸组和药物组大鼠膝关节软骨积分明显低于模型组(均P<0.05),温针灸组积分低于药物组(P<0.05);在关节形态的micro-CT观察方面,温针灸组在缓解关节退变、促进关节软骨修复方面优于药物组。结论:温针灸不仅可以有效缓解关节疼痛,同时可以促进关节软骨修复,是一种安全有效的疗法。

Objective To observe the effects of warm needling moxibustion on body mass,knee cartilage and morphology in rats with knee osteoarthritis（KOA）.Methods Forty SD rats were randomly divided into a normal group,a model group,a medication group and a warm needling group,10 rats in each one.Except the normal group,the rats in the remaining three groups were injected with papain to establish the model of KOA.After the modeling,rats in the model group did not receive any treatment;rats in the warm needling group were treated with warm needling moxibustion at bilateral＂Xiqian＂;rats in the medication group were treated with intragastric administration of meloxicam;rats in the normal group were treated with 0.9% NaCl solution（identical dose as medication group）and immobilized as the warm needling group.The treatment was given once a day for consecutive 20 days.The body mass,scale of knee cartilage and morphological changes were observed in each group after treatment.Results The increasing of body mass in the medication group and warm needling group was faster than that in the model group,but slower than that in the normal group（all P0.05）;the difference between medication group and warm needling group was not statistically significant（P0.05）.The scale of knee cartilage in the warm needling group and medication group was significantly lower than that in the model group（both P0.05）,while the scale in the warm needling group was lower than that in the medication group（P0.05）.Regarding the knee morphology under micro-CT,the relief of knee degeneration and improvement of knee recovery in the warm needling group were superior to those in the medication group.Conclusion The warm needling moxibustion could effectively reduce the knee pain,improve the recovery of knee cartilage,which is a safe and effective treatment.