Sensititre结核药敏板检测结核分枝杆菌药敏的方法学研究

Methodological study of Sensititer tuberculosis drug sensitive plate in detecting mycobacterium tuberculosis

目的评价使用Sensititre结核药敏板进行结核分枝杆菌药敏试验的价值。方法分别使用Sensititre结核药敏板和L-J比例法对124株结核分枝杆菌复合群的临床分离株进行药敏试验(包括异烟肼、利福平、乙胺丁醇、链霉素、氧氟沙星、莫西沙星、阿米卡星、利福布汀、对氨基水杨酸、乙硫异烟肼、环丝氨酸、卡那霉素),并将两法结果进行比较。结果使用Sensititre结核药敏板和L-J比例法对124株结核分枝杆菌复合群的临床分离株进行异烟肼、利福平、乙胺丁醇、链霉素、氧氟沙星、莫西沙星、阿米卡星、利福布汀、对氨基水杨酸、乙硫异烟肼、环丝氨酸、卡那霉素等12种药物的敏感性试验。其敏感度、特异度均较好。各药的符合率都达到了90.0%以上。结论使用Sensititre结核药敏板所得的药敏结果与传统的L-J比例法有较高的一致性,且其检测时间较短、操作更为简单,同时还能获得抗结核药物的最低抑菌浓度值。

Objective The present study is designed to evaluate Sensititre tuberculosis drug sensitive plat in testing antituberculotics sensitivety to mycobacterium tuberculosis.Methods To assess the effectiveness of Sensititre tuberculosis drug sensitive plat,the sensitivety of different antituberculotics（isoniazid,rifampicin,ethambutol,streptomycin,levofloxacin, moxifloxacin, Amikacin, rifabutin, para aminosalicylic acid, ethionamide,cycloserine,kanamycin）was tested on 124 strains of Mycobacterium tuberculosis complex from clinical isolates by Sensititre tuberculosis drug sensitive plat or L-J propotion method.Results The sensitivity and specifity of Sensititre tuberculosis drug sensitive plat in testing the antituberculotics sensitivety were about 90.0% or more,which was compared with the L-J proportion method.Conclusion The effectiveness of Sensititre tuberculosis drug sensitive plat was consistent with that from the L-J proportion method,but it shortened the test duration and was more convenient.In addition,Sensititre tuberculosis drug sensitive plat can get minimum inhibitory concentrations of the different antituberculotics.