CLIA法联合TPPA法在临床梅毒血清学筛查中的应用评价

Evaluation of CLIA application in combination with TPPA for clinical serological screening for syphilis

目的探讨化学发光免疫分析(CLIA)法联合梅毒螺旋体明胶颗粒凝集试验(TPPA),在临床梅毒血清学筛查中的应用价值。方法用CLIA进行特异性梅毒螺旋体抗体初筛试验,阳性标本再经TPPA和梅毒甲苯胺红不加热血清试验(TRUST)复检。CLIA与TPPA结果不一致者用蛋白印迹法(WB)验证。结果 599例CLIA初筛阳性标本用TPPA法复检阳性568例、阴性31例;TRUST法复检阳性189例、阴性410例。31例TPPA阴性标本经WB试验验证结果为18例阴性、13例阳性。结论 CLIA法联合TPPA法初筛梅毒作为梅毒筛查的流程是可行的,值得推广。

Objective To explore the application value of chemiluminescent immunoassay（CLIA）in combination with treponema pallidum particle agglutination（TPPA）for clinical serological screening for syphilis.MethodsCLIA method was used to screen specific treponema pallidum antibody.The positive samples were then retested by the methods of TPPA and syphilis toluidine red unheated test（TRUST）.The samples with inconsistent results obtained from CLIA and TPPA were retested by Western blot（WB）.Results The 599 patients screened as positive by CLIA were retested by TPPA,with the results of 568 positive and 31 negative samples,then retested by TRUST,with the results of 189 positive and 410 negative samples.WB was used to confirm the 31 negative samples by the method of TPPA,with the results of 13 positive samples and 18 negative samples.Conclusion The combination of CLIA and TPPA methods for syphilis serological screening is feasible and worth promotion.