冬连胶囊对STZ诱导糖尿病肾病大鼠p38MAPK、CREB1及TGFβ1蛋白表达的影响

Effect of Donglian Capsules on Protein Expression of p38MAPK,CREB1 and TGFβ1 in Diabetic Nephropathy Model Rats Induced by Streptozotocin

目的:探讨冬连胶囊对链脲佐菌素(STZ)诱导的糖尿病肾病大鼠肾脏保护作用及TGF-β1/p38MAPK信号通路影响。方法:雄性SD大鼠60只,随机分为正常组10只,模型组50只。模型组大鼠腹腔注射链脲佐菌素(STZ)(45 mg/kg),造模成功的大鼠按体质量随机分为模型组、科素亚组、冬连胶囊低、中、高剂量组。分别于灌胃后第1、3、8周收集24 h尿液检测24 h尿蛋白定量。灌胃第8周,处死大鼠取肾组织,western-blotting法观察转化生长因子β1(TGF-β1)、p38MAPK、CREB1蛋白表达。结果:与模型组比较,各治疗组肾脏组织中TGF-β1、p-p38MAPK、p-CREB1,蛋白表达明显减少(P<0.01);冬连胶囊各治疗组可不同程度降低模型大鼠的24 h尿蛋白量,减轻模型大鼠的肾比重,对糖尿病肾病模型大鼠具有肾脏保护作用。结论:冬连胶囊可能通过调控糖尿病肾病肾组织TGF-β1/p38MAPK/CREB1信号表达,减轻肾脏细胞纤维化、减缓肾小球细胞硬化,进而降低24h尿蛋白定量,减轻肾脏损害,对糖尿病肾病起到一定的保护及延缓病程进展的作用。

Objective： To discuss the protective effect of Donglian capsules on SD rats induced by STZ and its influence on TGF-β1 / p38 MAPK signal path. Methods： Sixty SD male rats male were divided into two groups：a normal group with 10 rats and a model group with 50 rats. The rats in the model group were injected by abdominal Streptozotocin（ STZ）（ 45 mg / kg）. After successful models,50 model rats were randomized into five groups： a model group,a Cozaar group,a Donglian low-dose group,a Donglian middle-dose group and a Donglian high-dose group. Collect 24 h urines and test 24 h urinary protein on 1,3,8 weeks after lavage. On the 8th week of lavage,kill the rats and get out kidney tissues to observe the protein expressions of TGF-β1,p38 MAPK and CREB1 by western-blotting method. Results： Compared with the model group,TGF-β1,p38 MAPK and CREB1 protein expressions of the treatment group in the kidney tissues decreased significantly（ P〈 0. 01）; Donglian capsules treatment groups could reduce 24 h urinary protein quantity of the STZ model rats to different degrees,and it could reduce the kidney proportion of model rats and protect kidney of diabetic nephropathy model rats. Conclusion： Donglian capsules can regulate TGF-β1 / p38 MAPK / CREB1 signal representation in kidney tissues of diabetic nephropathy,relieve renal fibrosis cells,reduce glomerular sclerosis of cells and thus reduce the 24 h urinary protein quantity,then relieve kidney damage,and it can play a protective role and delay the progression of diabetic nephropathy.