交泰丸对db/db小鼠脂肪肝防治作用的实验研究

Experimental Study on Prevention Effect of Jiaotai Wan( 交泰丸)on db / db Mice from Fatty Liver

目的:研究交泰丸对db/db小鼠脂肪肝的治疗作用。方法:18只db/db小鼠分为模型组、交泰丸组、罗格列酮组,另取db/db鼠同窝的db/m小鼠设为正常组。各组灌胃给药4 W,正常组与模型组给予生理盐水。观察小鼠的进食量、饮水量、体质量、空腹血糖(FBG)、血脂的变化,光镜观察肝组织病理学改变。Western-bloting检测ACC(乙酰辅酶A羧化酶)、p-ACC的表达量变化。结果:交泰丸组的肝脏脂肪变性轻于模型组;罗格列酮组和交泰丸组p-ACC表达量较模型组有明显增加。结论:交泰丸有较好的防治糖尿病脂肪肝的作用。

Objective： To study the prevention effect of Jiaotai Wan on db / db mice from fatty liver disease.Methods： db / db mice were randomly divided into model group,Jiaotai Wan group,Rosiglitazone group,db / m mice were taken as normal control group. Each group was given the corresponding therapy for 4 weeks,mice in normal group and model group were received only physiological saline. During this period,body weight,water intake,food- intake,fasting blood glucose（ FBG）,blood fat were determined,pathological changes of liver were observed by light microscope,ACC and p- ACC expressions were determined by Western- bloting. Results：Compared with model group,hepatic steatosis of Jiaotai Wan group was lighter,p- ACC exprestion of Jiaotai Wan group and rosiglitazone group were significant increased. Conclusion： Jiaotai Wan is effective for the prevertion of fatty liver in db / db mice.