杨梅树皮氯仿萃取物抗小鼠Lewis肺癌的实验研究

Inhibition Effect of Chloroform Extracts from Yangmei( Myrica Rubra)Bark on Lewis Lung Cancer of Mice

目的：观察杨梅树皮氯仿萃取物对C 57BL/6J小鼠Lewis肺癌的抑制作用及对肿瘤坏死因子（TNF-α）、基质金属蛋白酶-9（MMP-9）、基质金属蛋白酶抑制剂-1（TIMP-1）表达的影响。方法：建立C 57BL/6J小鼠Lewis肺癌模型,分别设杨梅树皮氯仿萃取物高剂量组（13.2g生药/kg）、中剂量组（6.6 g生药/kg）、低剂量组（3.3 g生药/kg）、环磷酰胺（CTX）阳性对照组（30 mg/kg×3）及模型对照组（水30 m L/kg）,给药12天后处死动物,称瘤重,计算抑瘤率（%）;ELISA法测定血清TNF-α、MMP-9、TIMP-1含量。结果：杨梅树皮氯仿萃取物对C57BL/6J小鼠Lewis肺癌有一定的抑制作用,抑瘤率在16.3%~41.6%,其中高、中剂量组的瘤重与模型组比较有显著性差异（P〈0.05~0.01）;同时能明显增高血清TNF-α、TIMP-1表达,降低MMP-9表达。结论：杨梅树皮氯仿萃取物对C 57BL/6J小鼠Lewis肺癌有较好的抑制作用,且能上调TNF-α、TIMP-1表达,下调MMP-9表达。

Objective： To observe the effect of chloroform extracts from Myrica rubra bark on Lewis lung cancer of mice and impact on TNF- α,MMP- 9,TIMP- 1 expressions. Methods： Lewis lung cancer models of C57 BL /6J mice were established. Mice were divided into five groups： high（ 13. 2 g / kg）,middle（ 6. 6 g / kg）and low（ 3. 3 g / kg） dose of chloroform extracts from bark of Myrica rubra,CTX group（ 30 g / kg × 3） and model group（ H2 O 30 m L / kg）. Mice were sacrificed after 12 days. According to the final tumor weight,the inhibition rate was calculated. The expressions of TNF- α,MMP- 9 and TIMP- 1 were inspected by ELISA method.Results： Chloroform extracts from bark of Myrica rubra had certain inhibitory effect on Lewis lung cancer in mice and the inhibition rate was between 16. 3% to 41. 6%. The tumor weight of high and middle dose groups were smaller than that of model group（ P 0. 05 ~ 0. 01）. Chloroform extracts can also significantly increase the expressions of TNF- α and TIMP- 1,decrease the expression of MMP- 9. Conclusion： Chloroform extracts from bark of Myrica rubra had good inhibitory effect on Lewis lung cancer in C57 BL /6J mice,and can also increase the expression of TNF- α and TIMP- 1,lower the expression of MMP- 9.