乳腺浸润性导管癌组织中EpCAM、Vimentin、N-Cadherin的表达观察

Expression of Ep CAM,vimentin and N-cadherin in breast infiltrating ductal carcinoma

目的观察乳腺浸润性导管癌组织中上皮细胞黏附分子(Ep CAM)、波形蛋白(Vimentin)、N-钙黏附蛋白(N-Cadherin)的表达变化,并探讨其意义。方法 835例份乳腺浸润性导管癌组织(观察组),其中腺腔A型229例份(A组)、腺腔B型[人类表皮生长因子受体2(HER2)阴性]263例份(B组)、腺腔B型(HER2阳性)92例份(C组)、HER2过表达型90例份(D组)、三阴型161例份(E组),另取60例份乳腺良性病变组织作对照(对照组),检测各组Ep CAM、Vimentin、N-Cadherin,并分析三者表达与乳腺浸润性导管癌临床病理参数的关系。结果观察组中Ep CAM、Vimentin、N-Cadherin阳性率分别为53.4%、11.4%、9.7%,对照组分别为11.7%、0、0,两组比较,P均<0.05;A、B、C、D、E组Ep CAM阳性率两两比较(除D组分别与B、E组比较外),A组Vimentin阳性率分别与B、C、D、E组比较,B组Vimentin阳性率分别与E组比较,E组N-Cadherin阳性率与A、B组比较,B组N-Cadherin阳性率分别与C、D组比较,P均<0.05。Ep CAM、Vimentin、N-Cadherin表达均与乳腺浸润性导管癌肿瘤直径、淋巴结转移、组织学分级、ER、PR、临床分期有关,而且Ep CAM与HER2有关(P均<0.05)。观察组中Ep CAM与Vimentin、N-Cadherin表达呈正相关(r=0.214、0.136,P均<0.05),A、C、E组中Ep CAM与Vimentin表达呈正相关(r=0.301、0.290、0.270,P均<0.05),C、E组中Ep CAM与N-Cadherin表达呈正相关(r=0.003、0049,P均<0.05)。结论乳腺浸润性导管癌组织中Ep CAM、Vimentin、N-Cadherin表达升高,尤其在三阴型中呈高表达,其可能参与该型乳腺浸润性导管癌的发展、转移过程。

Objective To observe the expression change~ of vimentin, N-cadherin, epithelial cell adhesion molecule （EpCAM） in breast infiltrating ductal carcinoma, and to investigate their clinical significance. Methods Totally 835 ca- ses of breast infiltrating ductal carcinoma tissues （observation group） were divided into 229 cases of luminal A （group A）, 263 cases of luminal B [ hman epidermal growth factor receptor-2 （HER2） - ] （ group B）, 92 cases of luminal B （ HER2 ＋ ） （group C）, 90 cases of HER-2 with overexpression （group D） and 161 cases of triple-negative subtype （group E）. Meanwhile, another 60 cases of benign breast lesions （control group） were also studied. The expression of EpCAM, vim- entin and N-cadherin was detected in the observation group and the control group, and their relationships with the clinical- pathological parameters of breast infiltrating ductal carcinoma were analyzed. Results The positive expression rates of Ep- CAM, vimentin and N-cadherin in the observation group were 53.4%, 11.4% and 9.7% respectively, and those in the control group were 11.7%, 0, 0, respectively. Significant differences were found between the observation group and con- trol group （ all P 〈 0.05 ）. The differences were significant in the following comparisons ： the muhiple comparisons among EpCAM expression of groups A, B, C, D and E （except for D vs B and D vs E） ; the vimentin expression of group A as compared with that of groups B, C, D and E respectively; the vimentin expression of group B as compared with that of group E ; the N-cadherin expression of group E as compared with that of groups A and B respectively; ,the N-cadherin ex- pression of group B as compared with that of group C and D （ all P 〈 0.05）. The expression of EpCAM, vimentin and N- cadherin was associated with tumor diameter, lymph node metastasis, histological grade, ER, PR and TNM stage （all P 〈 0.05） ; in addition, the expression of EpCAM was also related with HER2 （P 〈 0.05）. The expression of EpCAM in the observation group was positively correlated with the expression of vimentin and N-cadherin （r = 0. 214, 0. 136; all P 〈 0. 05 ）. EpCAM expression was positively correlated with vimentin expression in the groups A, C and E （ r =0.301,0.290 and 0.270, respectively; all P 〈 0. 05）, and EpCAM expression was positively correlated with N-cadherin expression in the groups C and E （r=0.003,0049; all P〈0.05）. Conclusions The expression of EpCAM, vimentin and N-cadherin increases in the breast infiltrating ductal carcinoma tissues, especially in the triple-negative subtype, which may be involved in the development and metastasis of this type of breast infiltrating ductal carcinoma.