酵母菌发酵有机米糠富锗的条件优化

Optimization of germanium accumulation conditions by yeast to ferment the organic rice bran

试验以有机米糠为发酵底物,研究酵母菌对锗的生物富集作用,通过对富锗酵母生物量以及锗含量的测定,对此发酵试验的条件进行优化。试验结果显示:令初始p H值自然,米糠添加量32 g/250 ml、装液量80 ml/250 ml、接种量10%、时间60 h、温度30℃、GeO_2添加量为100 mg/l时,优化培养条件所得的富锗酵母菌发酵的有机米糠的有机锗生产水平为11.83 mg/kg。

The experiment took the organic rice bran as the fermentation substrate to study the biologi-cal accumulation function of the yeast to germanium. The biomass and germanium- enriched yeastwere measured for optimizing the experiment conditions. The result showed： under the optimized condi-tions that the initial p H value was unchanged, the filling volume of organic rice bran was 32 g/250 ml,the filling volume of liquid medium was 80 ml/250 ml, the inoculum was 10%（v/v）, the cultural timewas 60 h, the cultural temperature was 30 ℃, Ge O2 concentration was 100 mg/l, the organic germani-um production-level of the organic rice bran fermented by yeast was 11.83 mg/kg.