青藏高原阿旺绵羊线粒体基因组研究

Study and Analysis on Complete Mitochondrial Genome Sequence of Awang Sheep of the Qinghai-Tibetan Plateau

利用重测序方法对青藏高原阿旺绵羊线粒体基因组进行测序、组装,并对基因特点和系统发育进行分析。结果表明:阿旺绵羊线粒体基因组全长16 618 bp,该基因由13个蛋白编码基因、22个转运RNA基因、2个核糖体RNA基因和1个非编码控制区组成;基因特点显示碱基A含量33.69%、T含量27.40%、C含量25.82%、G含量13.09%,A+T含量(61.09%)比G+C含量(38.91%)高,表现出一定的碱基偏好,转换多于颠换,表现较高的转换偏向;基于线粒体基因组构建了31个不同物种的NJ树,表明阿旺绵羊与绵羊属亲缘关系最近,与牛属和岩羊属亲缘关系最远。

Awang sheep is a very important local breed of the Qinghai- Tibetan plateau in China.In this experiment,the complete mitochondrial genome sequence of Awang Tibetan sheep were studied for the first time,the results showed that the total length of the mitogenome was 16 618 bp,consisting of 13 protein- coding genes,22 transfer RNA（t RNA） genes,two ribosomal RNA（r RNA）genes,and a non- coding control region（D- loop region）.As in other mammals,its most mitochondrial genes were encoded on the heavy strand. Its overall base composition was A：33.69%,T：27.40%,C：25.82%,and G：13.09%,A＋T（61.09%）was higher than G＋C（38.91%）. The phylogenetic relationship indicated that the Awang sheep was close with the Ovis aries.