摘要
目的探讨AGEs类物质在不同程度的糖尿病慢性肾脏疾病(CKD)患者体内的蓄积情况。方法选取糖尿病患者259例,根据24hUAlb分为单纯糖尿病(DM,UAlb300mg/24h)组53例、肾功能失代偿(CKD3,Scr 133-442μmol/L)组51例及尿毒症(U,Scr〉442μmol/L,持续血液透析治疗)组35例。另选同期体检健康者51名作为对照(NC)组。检测各组血清中AGEs、低分子量AGEs(LMWAGEs)和游离戊糖苷酶(FP)的荧光强度。结果 CKD1组血清AGEs荧光强度高于DM组和NC组,且DM组高于NC组[(14.94±3.07)vs(10.80±2.35)vs(6.23±1.25)U/mg],CKD3组高于CKD2组[(51.50±10.33)vs(15.57±3.20)U/mg](P0.05),其余各组两两比较差异均有统计学意义(P〈0.05),且U组最高;U组血清FP荧光强度高于其他各组(P=0.001),而其余各组两两比较,差异无统计学意义。结论测定血清AGEs和LMW-AGEs的水平可对早期肾损害的诊断、肾损害程度的分析及终末期肾病的评价提供帮助,FP主要适用于终末期肾病的评价,而对早期肾损害不敏感。
Objective To investigate the accumulation of advanced glycation end products(AGEs)in patients at different phases of chronic kidney disease(CKD)in diabetes. Methods A total of 259 cases of diabetes(DM)were divided into five groups according to the levels of 24halbuminuria(UAlb):simple diabetes group(DM,n=58,UAlb30mg/24h),microalbuminuria group(CKD1,n=62,UAlb 30-300 mg/24h),clinical protein urine group(CKD2,n=53,UAlb〉300 mg/24h),nitremia group(CKD3,n=51,Scr 133-442μmol/L)and uremia group(U,n=35,Scr〉442μmol/L,haemodialysis).51 healthy volunteers served as normal controls(NC group).The fluorescent intensity of AGEs,LMW-AGEs and free pentosidine(FP)in the serum were determined. Results Fluorescent intensity of AGEs in serum:CKD1group was higher than DM group and NC group and DM group was higher than NC group[(14.94±3.07)vs(10.80±2.35)vs(6.23±1.25)U/mg];CKD3group was higher than CKD2group[(51.50±10.33)vs(15.57±3.20)U/mg](P〈0.05).Fluorescent intensity of LMW-AGEs in serum:there was no statistically significant difference between group DM and group CKD1,CKD2 and CKD3(P〈0.05);the remaining differences between two groups were statistically significant(P〈0.05)with U group was the highest.Fluorescent intensity of FP in serum:U group was higher than other groups(P=0.001).The difference was not statistically significant in the rest of pairwise group comparison(P〈0.05). Conclusion It would be valuable to measure the serum levels of AGEs and LMW-AGEs in screening of early nephropathy and evaluating the extent of renal damage.Monitoring the level of FP is sensitive to the evaluation of end-stage nephropathy but not in early-stage nephropathy.
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2016年第2期127-130,共4页
Chinese Journal of Diabetes
关键词
糖尿病慢性肾脏疾病
非酶糖化终末产物
荧光强度
Chronic kidney disease(CKD)in diabetes
Advanced glycation end products(AGEs)
Fluorescent intensity