摘要
目的探讨结肠癌转移相关基因1(MACC1)和肝细胞生长因子受体蛋白(c-Met)表达与宫颈癌盆腔淋巴结转移相关性。方法选取宫颈癌患者113例,宫颈上皮内瘤变Ⅱ~Ⅲ患者41例,正常宫颈组织患者47例,利用免疫组化SP法检测MACC1和c-Met在组织中表达,利用二分类Logistic回归分析对宫颈癌盆腔转移相关因素进行分析。结果 MACC1和c-Met在宫颈癌组织中相对表达量分别为(7.64±1.37)和(6.73±1.22),均高于宫颈上皮内瘤变Ⅱ~Ⅲ组织和正常宫颈组织,且宫颈上皮内瘤变Ⅱ~Ⅲ组织高于正常宫颈组织,差异均有统计学意义(P〈0.05);Spearman相关分析显示,MACC1在宫颈癌组织中表达与c-Met呈正相关(r=0.467,P〈0.05);宫颈癌发生盆腔淋巴结转移与脉管浸润、肌层浸润深度、MACC1阳性表达、cMet阳性表达有关(P〈0.05),多因素Logistic回归分析显示,肌层浸润深度、MACC1阳性表达和c-Met阳性表达是宫颈癌发生盆腔淋巴结转移的独立危险因素(P〈0.05)。结论 MACC1和c-Met在宫颈癌组织中呈高表达,可能共同参与了宫颈癌发生及进展,是宫颈癌发生盆腔淋巴结转移的独立危险因素。
Objective: To explore the correlation between metastasis-associated in colon cancer-1 (MACC1), hepatocyte growth fac- tor receptor protein (c-Met) and cervical lymph node metastasis of cervical cancer. Methods: A total of 113 cervical cancer patients, 41 cervical intraepithelial neoplasia (CIN) Ⅱ-Ⅲ patients, and 47 cases with normal cervical tissue were selected. The expressions of MACC1 and c-Met were detected by immunohistochemical SP method. The related factors of cervical cancer pelvic metastasis were analyzed by binary Logistic regression analysis. Results: The relative expression levels of MACC1 and c-Met in cervical cancer were (7. 64±1.37) and (6.73±1.22), respectively, which were higher than those in CIN Ⅱ-Ⅲ tissue and normal cervical tissue, the relative expression levels of MACC1 and c-Met in CIN Ⅱ-Ⅲ tissue were higher than those in normal cervical tissue, there were statistically significant differences (P〈 0. 05) . Spearman correlation analysis showed that the expression of MACC1 was positively correlated with the expression of c-Met in cervi- cal cancer (r = O. 467, P〈O. 05) . Cervical cancer pelvic lymph node metastasis was correlated with vascular invasion, depth of myometrial invasion, MACC1 positive expression, and c-Met positive expression (P〈0. 05) . Multivariate Logistic regression analysis showed that the depth of myometrial invasion, MACC1 expression, and c-Met expression were independent risk factors of cervical cancer pelvic lymph node metastasis (P〈0.05) . Conclusion: The expressions of MACC1 and c-Met are high in cervical cancer, they might be involved in the oc- currence and progression of cervical cancer, which are independent risk factors of cervical cancer pelvic lymph node metastasis.
出处
《中国妇幼保健》
CAS
2016年第4期838-841,共4页
Maternal and Child Health Care of China
关键词
宫颈癌
盆腔淋巴结
结肠癌转移相关基因1
肝细胞生长因子受体蛋白
相关
Cervical cancer
Pelvic lymph node
Metastasis-associated in colon cancer-1
Hepatocyte growth factor receptor
Corre-lation