高效液相色谱法测定大花红景天野生与组培品种中5种主要活性成分

Simultaneous Determination of Five Active Compounds in Wild and Culture Materials of Rhodiola crenulata by RP-HPLC

目的建立高效液相色谱法测定大花红景天的野生品种、愈伤组织、组培苗及与真菌共生苗中5种主要活性成分的方法。方法采用Thermo-C_(18)柱(4.6 mm×250 mm,5μm),流动相为甲醇-水(32∶68)洗脱,进样量10μL,体积流量0.8 m L·min^(-1),柱温30℃,分段检测波长:0~13 min 277 nm、13~60 min 250 nm。结果红景天苷、酪醇、络塞琳、洛塞维和络塞分别在2.80~280.00μg·m L^(-1)(r=0.999 8)、2.80~280.00μg·m L^(-1)(r=0.999 7)、1.20~120.00μg·m L^(-1)(r=0.999 6)、1.60~160.00μg·m L^(-1)(r=0.999 7)、1.20~120.00μg·m L^(-1)(r=0.999 7)与峰面积呈良好线性关系,加样回收率为99.32%~100.45%。结论该方法用于大花红景天的野生、愈伤组织、组培等材料的含量测定提供方便、可靠的手段。

OBJECTIVE To establish a method for simultaneous determination of five components in wild and tissue-culture mate- rials of Rhodiola crenulata. METHODS The determination was performed on a Thermo-C18 column （4. 6 mm × 250 mm,5 μm） with mobile phase composed of methanol-water（32： 68） at a flow rate of 0. 8 mL·min^-1. The injection volume was 10 μL, column temperature was set at 30℃, and the detection wavelength was set at 277 nm at 0 - 13 min and 250 nm at 13 -60 min. RESULTS There were linear relationships between the peak areas and contents of salidroside, p-tyrosol, rosarin, rosavin and rosin in the ranges of 2. 80-280.00（r =0.999 8）, 2.80-280.00（r =0.999 7）, 1.20- 120.00（r =0.999 6）, 1.60 -160.00（r =0.999 7） and 1.20 - 120. 00 μg·mL^-1 （r = 0. 999 7 ）, respectively. The extraction recoveries varied from 99. 32% to 100. 45 %. CONCLUSION The established method is simple and accurate for quality control of wild and tissue-culture materials of Rhodiola.