缩泉胶囊HPLC指纹图谱与组方药材相关性

HPLC Fingerprint of Suoquan Capsules and Correlativity with the Relevant Herbs

考察了缩泉胶囊与山药、益智仁、乌药3味组方药材指纹图谱的相关性,为缩泉胶囊指纹图谱的检测标准提供参考。采用高效液相色谱法,使用Agilent Zorbax SB-C_（18）色谱柱,以乙腈∶0.1%磷酸为流动相,梯度洗脱,检测波长280 nm（0~21 min）、260 nm（21~33 min）和230 nm（33~50 min）。结果 10批样品共标定14个共有峰,样品指纹图谱相似度均大于0.90,缩泉胶囊与组方药材间指纹图谱的相关性良好。

The chemical correlations between Suoquan capsules and the relevant herbs, Rhizoma Dioscoreae, Fructus Alpiniae Oxyphyllae and Radix Linderae were evaluated by HPLC fingerprint analysis to offer a theoretical basis for the fingerprint detecting standard of Suoquan capsules. An Agilent Zorbax SB-C_（18） column was used, with the mobile phase of acetonitrile∶0.1% phosphoric acid by gradient elution, at the detection wavelengths of 280 nm（0—21 min）, 260 nm（21—33 min） and 230 nm（33—50 min）. There were 14 common peaks in the HPLC fingerprints of 10 batches of samples, and their similarities were all over 0.90. The results showed a consistency between Suoquan capsules and their relevant herbs.