摘要
目的研究补肾和脉方(HMF)对载脂蛋白E基因敲除(Apo E^(-/-))小鼠动脉粥样硬化(AS)的治疗效果及可能机制。方法将24只Apo E^(-/-)小鼠随机分为高脂组、HMF组和阿托伐他汀组,每组8只。高脂组给予高脂饲料(0.25%胆固醇和15%可可脂)喂养,HMF组给予高脂饲料+HMF[1.37 g/(kg·d)]喂养,阿托伐他汀组给予高脂饲料+阿托伐他汀[5 mg/(kg·d)]喂养。8周后,戊巴比妥钠(0.8%,0.05 m L/10 g)腹腔注射麻醉,取小鼠胸主动脉切片,行常规HE染色观察胸主动脉斑块大小;行免疫组化染色检测巨噬细胞CD68、肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β和IL-8的表达水平。并采用日本全自动生化分析仪测量小鼠总胆固醇(TC)和总三酰甘油(TG)水平。结果 HMF组胸主动脉斑块大小,胸主动脉斑块内CD68、TNF-α、IL-1β和IL-8的表达水平,TC、TG水平均明显低于高脂组,高于阿托伐他汀组(均P<0.05)。结论 HMF具有抗动脉硬化作用,可抑制AS进展,其机制可能与降低血脂,减轻斑块炎症细胞浸润,减少炎症因子分泌有关。
Objective To observe the therapeutic effect and mechanism of Bu-shen-he-mai-fang （HMF） on experi- mental atherosclerosis （AS） in apolipoprotein-E knockout （ApoE-/-） mice. Methods Twenty-four male ApoE-/- mice were randomly divided into three groups including high-fat group （0.25% cholesterol and 15% cocoa butter）, HMF group [（high- fat diet ＋ HMF decoction 1.37 g/（kg, d）] and atrovastatin group [（high-fat diet ＋ atrovastatin 5 mg/（kg, d）], 8 mice for each group. The serum level of lipid was evaluated by a kit. The extent of AS was evaluated by HE staining. The macrophage infil- tration and expression of tumor necrosis factor-α（TNF-α）, interleukin-1β （IL-1β） and interleukin-8 （IL-8） were evaluated by immunohistochemical staining. Results The serum level of lipid was lower in HMF group and atrovastatin group than that in high-fat group （P 〈 0.05）. The degree of AS was significantly lower in HMF group and atrovastatin group than that in high-fat group. The macrophage infiltration, TNF-α, IL-113 and IL-8 expressions were significantly lower in HMF group and atrovastatin group than those in high-fat group （P 〈 0.05）. Conclusion The results suggest that HMF significantly in- hibits early atherosclerotic lesions by lowering serum lipid level and inhibiting inflammatory response.
出处
《天津医药》
CAS
2016年第2期185-187,I0002,共4页
Tianjin Medical Journal
基金
国家中医药管理局资助课题(JDZX2012113)
