孕期脂多糖刺激对子代大鼠胰岛素抵抗的影响

Effects of prenatal exposure to lipopolysaccharide on insulin resistance in offspring rats

目的探讨孕期脂多糖(LPS)对子代成年大鼠胰岛素抵抗的影响。方法 SD孕鼠20只随机分为2组,每组10只。LPS组孕期第8、10、12天腹腔注射LPS 0.40 mg/kg,对照组腹腔注射无菌生理盐水0.5 m L。子鼠3月龄时测血糖、血胰岛素的浓度和瘦素(Leptin)水平,计算稳态胰岛素抵抗指数(HOMA-IR)和胰岛素敏感指数(QUICKI)。结果与对照组相比,3月龄LPS组子鼠血糖(mmol/L:7.72±0.42 vs 7.02±0.42)、血胰岛素(m IU/L:8.78±4.10 vs 1.51±0.27)、血Leptin水平(μg/L:3.88±1.40 vs 1.00±0.33)、HOMA-IR值(3.01±1.41 vs 0.47±0.09)明显高于对照组;LPS组QUICKI值明显低于对照组(0.57±0.07 vs 0.99±0.08)。结论孕期LPS刺激会引起子代大鼠胰岛素抵抗。

Objective To explore the effects of prenatal exposure to lipopolysaccharide （LPS） on insulin resistance in adult offspring rats. Methods Nulliparous, time-matched Sprague-Dawley rats were randomly divided into two groups （n= 10 for each group）： control group and LPS group. On the pregnant day 8, 10 and 12, rats in control group and LPS group were administered intraperitoneally with saline 0.5 mL or LPS 0.40 mg/kg, respectively. The blood levels of fasting plasma glucose （FPG）, insulin and Leptin were detected at 90 days of age in offspring. The steady state insulin resistance index （HOMA-IR） and insulin sensitivity index （QUICKI） were evaluated in adult offspring rats. Results Compared with the control group, blood levels of FPG （mmol/L： 7.72±0.42 vs 7.02±0.42）, insulin （mIU/L： 8.78±4.10 vs 1.51±0.27） and Leptin （μg/L： 3.88± 1.40 vs 1.00±0.33） were significantly increased in offsping of LPS group, as well as HOMA-IR （3.01±1.41 vs 0.47±0.09） in- creased, while QUICKI （0.57±0.07 vs 0.99±0.08） decreased in offsping of LPS-treated rats. Conclusion Prenatal exposure to LPS can result in abnormality of insulin resistance in offspring rats.