青霉素菌渣堆肥过程中青霉素钠降解菌的分离与鉴定

Isolation and Identification of a Penicillin-Degrading Strain during Composting of Penicillin Bacteria Residue

为了研究开发青霉素发酵菌渣堆肥资源化与无害化技术,采用传统的富集、分离、纯化等微生物学方法,在青霉素菌渣与猪粪混合堆肥过程中筛选出一株青霉素钠高效降解菌——PC-2,并对其进行形态表征和基于16S rRNA基因序列的微生物种属鉴定.结果表明：菌株PC-2属螯合球菌属（Chelatococcus sp.）,其能够利用青霉素钠为唯一碳源生长,但外加碳、氮源可显著提高菌株PC-2对青霉素钠的降解效率.当葡萄糖为碳源、蛋白胨为氮源、菌株PC-2接种量为14%、p H为6～8时,菌株PC-2在37℃下振荡培养6 h,对初始ρ（青霉素钠）为400 mg/L的青霉素钠的降解率可达98%以上.自堆肥过程中获取高效青霉素钠降解菌PC-2,预示着其在菌渣堆肥过程中的应用潜力,也有助于深入开展青霉素制药菌渣的安全有效与无害化处理处置方法的研究.

A penicillin-degrading strain PC-2 was isolated and purified from the compost of penicillin bacteria residue with pig manure. It was identified as Chelatococcus sp. via 16S rRNA gene based phylogenetic analysis and morphological observation. In addition,the results of PC-2 confirmed the ability to use penicillin G as its sole carbon source,although the supplementation of additional carbon and nitrogen sources could significantly increase the penicillin degradation efficiency. As high as 98% of penicillin was degraded by PC-2 after 6 h incubation under the optimal conditions of 37 ℃,14% inoculum size,pH 6-8 and 150 r/min,when the initial penicillin concentration was 400 mg/L and glucose and peptone were added as carbon and nitrogen sources,respectively. In conclusion,PC-2 has promising application potential in the composting process and provides fundamental information for safe and efficient penicillin bacteria residue treatment in the near future.