一起食物中毒病原菌斯坦利沙门菌的分子分型及耐药性分析

Molecular subtyping and antibiotic resistance analysis of Salmonella Stanley isolated from a foodborne disease outbreak

目的对引起跨区域食物中毒的斯坦利沙门菌分离株进行耐药性检测和分子分型分析,追溯并明确病因食品,为该菌防控工作提供科学依据。方法采用微量肉汤稀释法对15株斯坦利沙门菌进行18种抗生素敏感性试验,同时对菌株基因组DNA经限制性内切酶Xba I酶切后进行脉冲场凝胶电泳(PFGE)分子分型,并与数据库中其他沙门菌比较。结果此次疫情分离菌株中食品分离株和腹泻患者分离株血清型一致,耐药谱基本一致。经PFGE溯源分析后具有100%同源的PFGE带型,数据库中无相同带型的菌株。结论本起跨区域食物中毒是由斯坦利沙门菌污染的煮花生引起,分离菌株对红霉素耐药,对氯霉素处于中介,对其他抗生素敏感。

Objective To conduct the molecular subtyping and antibiotic resistance analysis of Salmonella Stanley isolated from a foodborne disease outbreak in Beijing and to identify the cause and implement effective prevention and control measures. Methods 15 strains were tested for antibiotic susceptibility and their DAN were digested by Xba I,compared with other strains in the database. Results All of the 15 strains isolated from foods and patients showed the same serological subtype,similar antibiotic resistance results and 100% homologous PFGE patterns which couldn＇t match to the PFGE database. Conclusion The multi-county foodborne disease outbreak was caused by Salmonella Stanley which were resistanted to ERY and sensitive to 16 antibiotics.