电针结合天麻素对局灶性脑缺血大鼠神经功能、额叶皮质勿动蛋白A及其受体表达的影响

Effect of Electroacupuncture Intervention Combined with Gastrodin Administration on Neurological Function,Nogo-A and NgR Expression in the Frontal Lobe Cortex of Focal Cerebral Ischemia Rats

目的:观察电针结合天麻素对局灶性脑缺血大鼠神经功能及缺血灶周围区额叶皮质勿动蛋白A(Nogo-A)及受体(NgR)表达的影响,探讨针药结合治疗脑缺血的部分神经再生机制。方法:SD大鼠随机分为正常组、模型组、电针组、天麻素组和电针+天麻素组,每组10只。采用线栓法复制局灶性脑缺血大鼠模型。电针组电针左侧"曲池"穴和"合谷"穴,刺激30min,每天1次,共治疗14d;天麻素组按10mg/kg剂量腹腔注射天麻素注射液,每天1次,共治疗14d;电针+天麻素组给予电针与天麻素治疗,每天1次,共治疗14d。观察大鼠治疗后神经功能恢复情况,免疫组织化学方法检测缺血灶周围区额叶皮质Nogo-A及NgR蛋白的表达。结果:电针组、天麻素组和电针+天麻素组神经功能评分低于模型组(P<0.05),电针+天麻素组神经功能评分低于电针组、天麻素组(P<0.05)。模型组Nogo-A及NgR的表达较正常组明显增强(P<0.05),电针组、天麻素组和电针+天麻素组Nogo-A及NgR的表达较模型组明显减弱(P<0.05),电针+天麻素组Nogo-A及NgR的表达较电针组或天麻素组明显减弱(P<0.05)。结论:电针与天麻素结合可下调缺血灶周围区额叶皮质Nogo-A及NgR的表达,改善神经功能,其作用优于单独电针或天麻素治疗。

Objective To observe the effect of electroacupuncture（EA）intervention combined with medication（Gastrodin）on changes of neurological function and expression of Nogo-A and Nogo-A receptor（NgR）in the frontal lobe cortex around the ischemic loci of focal cerebral ischemia（FCI）rats,so as to explore its mechanism underlying improvement of neuroregeneration of FCI.Methods Fifty male Sprague-Dawley rats were randomly divided into normal control,model,EA,medication and EA＋medication groups（n=10in each group）.The FCI model was induced by occlusion of the middle cerebral artery（MCAO）with thread embolus.EA was applied to the left＂Quchi＂（LI 11）and＂Hegu＂（LI 4）for 30 min,once daily for 14 days after MCAO.For rats of the medication group,Gastrodin（10mg/kg）was administrated by intraperitoneal injection,once daily for 14 days.The neurological impairment was assessed by Zea Longa’s scoring.The expression of Nogo-A and NgR in the frontal lobe cortex around the ischemic loci was detected by immunohistochemistry.Results In comparison with the normal control group,cerebrocortical Nogo-A and NgR expression levels of the model group were significantly increased（P〈0.05）.Compared with the model group,the Zea Longa’s score and Nogo-A and NgR expression levels were evidently down-regulated in the EA,medication and EA＋medication groups（P〈0.05）.The Zea Longa’s score and Nogo-A and NgR expression levels were significantly lower in the EA＋medication group than in the EA and medication groups（P〈0.05）.Conclusion EA intervention and Gastrodin administration can down-regulate cerebro-cortical Nogo-A and NgR protein expression in FCI rats,which may contribute to their action in improving neurological impairment.The effect of EA＋Gastrodin is better than simple EA or Gastrodin treatment.