艾灸刺激对腓总神经横断术后大鼠胃黏膜修复作用机制的影响

Effect of moxibustion stimulation on repair of gastric mucosal lesions in rats after peroneal neurotomy

目的:为探讨艾灸启动内源性保护信息对胃黏膜的保护效应与腓总神经通路的关系.方法:将48只SD大鼠随机分为正常组A组,模型组B组,艾灸模型组C组,艾灸模型手术组D组,每组12只.予C、D组腓总神经切断术,术后护理处理3 d.除A予以生理盐水灌胃外,其余各组用无水乙醇、阿司匹林灌胃制备胃黏膜损伤模型.C组、D组给予艾灸"足三里(ST36)"治疗,2次/d,连续治疗3 d.3 d后观察胃黏膜损伤指数(ulcerindex,UI);胃黏膜相关修复细胞因子,表皮生长因子(epidermal growth factor,EGF)、前列腺素2(prostaglandin E2,PGE2)检测;胃黏膜细胞凋亡指数(apoptosis index,AI)检测.结果:与C组相比,D组在病理切片,UI改变效果较差(P=0.014);血清与组织中胃黏膜细胞相关修复因子E G F、P G E2增加不明显(血清EGF:P=0.003,组织EGF:P=0.000;血清PEG2:P=0.000,组织PGE2:P=0.000);胃黏膜凋亡蛋白AI比例增加不明显(P=0.000).结论:艾灸刺激足三里穴可通过腓总神经途径传递信息,调节胃黏膜保护因子的释放,并调控细胞凋亡相关蛋白表达,从而达到修复胃黏膜的效应.

AIM： To explore the mechanism underlying the protective effect of moxibustion on gastric mucosal lesions by investigating the relationship between endogenetic information started by moxibustion and the peroneal nerve pathways. METHODS： Forty-eight SD rats were randomly and equally divided into four groups： a normal group （A）, a model group （B）, a gastric mucosal injury ＋ moxibustion group （C）, and a gastric mucosa injury ＋ moxibustion ＋ surgery group （D）. Groups C and D were given 3 days of recovery after reroneal neurotomy. Group A was given saline and the other 3 groups were administered absolute ethanol and aspirin to induce gastric mucosa injury. After that, moxibustion was applied on Zusanli （ST36） in groups C and D, twice a day for 3 days. Finally, gastric ulcer index （UI） was assessed by gastric biopsy, gastric mucosa repair related cytokines such as epidermal growth factor （EGF） and prostaglandin E2 （PGE2） were detected, and mucosal apoptosis index （AI） was evaluated.RESULTS： The improvement of UI was better in group C than in group D （P -- 0.014）. Compared with group C, serum and gastriclevels of EGF and PGE2 （EGF in serum P = 0.003, EGF in tissue P = 0.000; PGE2 in serum P = 0.002, PGE2 in tissue P = 0.000）, and mucosal AI （P = 0.000） did not significantly rise in group D. CONCLUSION： Moxibustion on Zusanli can transmit information by peroneal nerve pathways to regulate the release of gastric mucosal protective factors and the expression of apoptosis-related proteins, thus achieving the effect of repairing the gastric mucosa.