M_2和M_3型胆碱能受体在脾气虚大鼠小肠运动中的作用

Role of M_2 and M_3 Type Cholinergic Receptors in the Small Intestine of Rats with Spleen Qi Deficiency

目的:研究M2、M3型胆碱能受体表达与脾气虚小肠运动的关系。方法:20只SD大鼠按随机数字表法分为对照组和脾气虚模型组(模型组)各10只,模型组大鼠复制饮食不节加劳疲型脾气虚模型;小肠运动采用小肠推进率评价;ELISA法检测小肠组织Ach和c AMP含量,免疫组化法观察M2、M3型受体表达。结果:与对照组比较,模型组大鼠的小肠推进率显著下降,Ach含量无变化,M2受体表达明显上升,M3受体表达和c AMP水平显著下降。结论:脾气虚小肠运动减弱与M2、M3型受体表达异常有关。

Objective： To study the roles of M2 and M3receptors in the small intestine（ SI） movement of Spleen-Qi deficiency rats. Methods： 20 male SD rats were randomly divided in the control and Spleen-Qi deficiency（ model group）groups,10 rats in each group; Spleen-Qi deficiency model of improper diet and over-fatigue type was established; The SI propelling rate was used to valuate the SI motion; ELISA was used to measure the Ach and c AMP concentrations in SI tissue; Immohistochemistry was employed to test expressions of M2 and M3receptors. Results： compared with those in control,the SI propelling rate was significantly reduced,Ach concentration was not changed,M2 receptor expression was markedly increased. Both M3 receptor expression and c AMP concentration were significantly decreased in the model group.Conclusion： the reduction of the SI motion in Spleen-deficiency model rats was contributed to the abnormal expressions of M2 and M3receptor.