芪叶保肝饮对肝癌细胞增殖和凋亡作用的研究

Study of Qiye Baogan Decoction on Proliferation and Apoptosis of Hepatocellular Carcinoma Cells

目的:研究芪叶保肝饮(SLPLD)对肝癌细胞增殖和凋亡的作用。方法:研究SLPLD清除DPPH自由基的能力和抗氧化作用;研究不同剂量SLPLD对Hep G2细胞增殖与凋亡的作用;建立小鼠肿瘤模型,实验组灌胃SLPLD,至第30天时结束描绘移植瘤生长曲线、称量终末移植瘤质量并计算抑瘤率。结果:SLPLD清除DPPH自由基能力随剂量增加而升高,呈线性相关:R2=0.9947,IC50=15.74ul;SLPLD对Hep G2细胞增殖结果显示细胞数量随SLP着LD剂量增加而减少。流式结果显示随SLPLD使用剂量增加,Hep G2细胞凋亡率由9.23%逐渐增加到42.9%;小鼠肿瘤模型第30天时SLPLD实验组肿瘤体积与平均肿瘤重显著小于对照组,肿瘤抑制率为40.3%。结论:SLPLD这一新型中成药具有较强的抗氧化活性,能够抑制Hep G2肝癌细胞增长,促进其凋亡。

Objective： To study the role of Qi Ye Bao Gan Yin（ SLPLD） in liver cancer cell proliferation and apoptosis. Method： Study the SLPLD’s ability of DPPH scavenging and its antioxidant effect. Research the Effect of different doses SLPLD on appreciation of Hep G2 cells. Established the tumor model in mice. The experimental group was given SLPLD. Experiment were ended at the 30 thday. Depict the tumor growth curve,weighing the terminal tumor mass,and inhibition rate was calculated. Result： DPPH scavenging ability positively correlated with the dose of SLPLD,and showed. linear correlation R2= 0. 9947,IC50 = 15. 74 ul. SLPLD ’s effect on Hep G2 cell proliferation and apoptosis showed that in SLPLD experimental group,cell number and the doses of SLPLD were negatively correlated. In SLPLD group with SLPLD dose increase,Hep G2 cell apoptosis rate increasing from 9. 23% to 42. 9% gradually. 3At the 30 th day,SLPLD tumor volume in experimental group was significantly smaller than the control group. Tumor inhibition rate was40. 3%. Conclusion： The new medicine SLPLD shows a strong antioxidant activity,it can inhibit the growth of Hep G2 and promote its apoptosis.