广泛性焦虑障碍患者治疗前后血浆MicroRNA-34c表达水平研究

MicroRNA- 34c plasma levels of pre- and post- treatment in generalized anxiety disorder patients

目的研究广泛性焦虑障碍(GAD)患者治疗前后血浆MicroRNA-34c(MiR-34c)表达水平及其与临床特征的相关性,为揭示血浆MiR-34c表达在GAD中的作用提供依据,探索GAD诊断和预后可能的新标志物。方法将42例GAD患者设为研究组,40例健康志愿者设为对照组。统计研究组一般人口学资料、首次发病年龄、总病程、本次病程等临床资料及对照组的一般人口学资料。研究组常规应用帕罗西汀片有效治疗量治疗12周,于治疗前后采用汉密尔顿焦虑量表(HAMA)进行疗效评估。以实时荧光定量PCR(RT-PCR)技术检测研究组的MiR-34c的表达水平并与对照组比较。结果研究组汉密顿焦虑量表评分治疗后较治疗前有显著下降(t=43.82,P<0.01),血浆MiR-34c表达水平较治疗前显著升高(t=21.55,P<0.01),治疗前后MiR-34c表达水平均显著低于对照组(t=15.39、4.37,P<0.01)。研究组治疗前后血浆MiR-34c的表达水平与HAMA总分和总病程呈显著负相关(R=-0.52,-0.49,-0.42,-0.44,P<0.01),而与年龄、首次发病年龄、本次病程均无相关性。结论血浆MiR-34c可能参与广泛性焦虑障碍的发病机制,焦虑程度越严重,总病程越长,血浆MiR-34c的表达水平越低,而且其表达水平受帕罗西汀药物影响。因此血浆MiR-34c有潜力成为广泛性焦虑障碍的状态标记物,并为其潜在的治疗靶点提供了依据。

Objective To examine the expression levels of pre- and post- treatment plasma MicroRNA- 34c（ MiR- 34c） in generalized anxiety disorder（ GAD） patients and their relation to clinical features. Methods 42 patients with GADand40 healthy controls were enrolled.Basic demographics,first- episode age,total course and current course of research group and generalized demographic data of control were added up. The patients were treated with Paroxetine for 12 weeks. Hamilton Anxiety Scale（ HAMA） was used to assess the efficacy of Paroxetine and MiR- 34 c plasma levels were examined by real- time PCR. Results After treatment,HAMA score lowered more significantly（ t = 43. 82,P〈0. 01） and plasma MiR- 34 c expression level elevated（ t = 21. 55,P〈0. 01） compared with pretreatment in research group. Pre- and post-treatment,MiR- 34 c expression level were significantly lower in research than in control group（ t = 15. 39,4. 37,P〈0. 01）. Pre- and post- treatment,MiR- 34 c expression level were significantly negatively related to the total score of the HAMA and total course（ R =- 0. 52,- 0.49,- 0. 42,- 0. 44,P〈0. 01） and had no correlation to age,first- episode age and current course in research group. Conclusion Low plasma MiR- 34 c expression level may be related to the onset of GAD,the severer anxiety degree and the longer total course,the lower the plasma MiR- 34 c expression level becomes.