妊娠肝内胆汁淤积症患者血清SOCS3含量检测及其与胎盘中过氧化反应、凋亡过程关系的研究

Detection of serum SOCS3 contents in patients with intrahepatic cholestasis of pregnancy and its correlation with peroxidation response and apoptosis process in placenta

目的研究妊娠肝内胆汁淤积症患者血清中细胞因子信号传导负调控因子(SOCS)的含量并分析其与胎盘组织中过氧化反应指标、凋亡指标的相关性。方法选择2013年6月至2014年8月就诊和分娩的妊娠肝内胆汁淤积症患者50例作为观察组,将同期分娩的健康产妇50例作为对照组,采集血清并检测SOCS-3含量,采集胎盘组织并检测Survivin、Xiap、Bcl-2、细胞色素c氧化酶(CCO)、琥珀酸脱氢酶(SDH)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、血红素氧化酶-1(HO-1)、丙二醛(MDA)的mRNA含量。结果观察组患者血清中SOCS3含量低于对照组;观察组胎盘中Survivin、Xiap、Bcl-2、CCO、SDH的mRNA含量低于对照组,且与SOCS-3呈正相关;观察组胎盘组织中SOD、GSH-Px、CAT以及HO-1含量低于对照组,且与SOCS-3呈正相关;MDA含量高于对照组,且与SOCS-3呈负相关。结论妊娠肝内胆汁淤积症患者血清SOCS3含量异常降低,且可能通过参与胎盘中过氧化反应、凋亡过程的调节来造成疾病的发生。

Objective To study the serum suppressor of cytokine signaling（ SOCS） content of patients with intrahepatic cholestasis of pregnancy and analyze its correlation with peroxidation response index and apoptosis index in placenta tissue. Methods 50 cases of intrahepatic cholestasis of pregnancy treated and laboring in our hospital from June 2013 to August 2014 were enrolled as observation group; 50 cases of healthy parturient women laboring during the same period were enrolled as control group. Serum was collected and SOCS- 3 content was detected; placenta tissue was collected and mRNA contents of survivin,Xiap,Bcl- 2,CCO,SDH,SOD,GSH- Px,CAT,HO- 1 and MDA were detected. Results serum SOCS3 content of observation group was lower than that of control group. mRNA contents of survivin,Xiap,Bcl- 2,CCO and SDH in placenta of observation group were lower than those of control group and were positively correlated with SOCS- 3. SOD,GSH- Px,CAT and HO- 1 contents in placenta tissue of observation group were lower than those of control group and were positively correlated with SOCS- 3. MDA content was higher than that of control group and was negatively correlated with SOCS- 3. Conclusion Serum SOCS3 content of patients with intrahepatic cholestasis of pregnancy abnormally decreases and may cause occurrence of the disease through participating in the regulation of peroxidation response and apoptosis process in placenta.