摘要
目的探讨血浆游离DNA定量检测在胃癌诊断中的价值。方法收集33例胃癌患者、44例健康对照者血液标本,血浆微量DNA抽提试剂盒提取血浆游离DNA,实时荧光定量PCR检测血浆游离DNA含量,评价血浆游离DNA水平在胃癌的诊断中的价值。结果胃癌组血浆游离DNA水平高于对照组(P<0.001);胃癌敏感性和特异度分别72.7%和93.2%,ROC曲线下面积为0.902。TNMⅢ期患者血浆游离DNA水平高于Ⅰ/Ⅱ期患者(P<0.05)。结论血浆游离DNA定量检测对胃癌的诊断具有一定的临床意义。
Objective To explore the cell-free DNA in the plasma of patients with gastric cancer and evaluate its diagnostic value. Methods The plasma cellfree DNA of 33 patients with gastric cancer and 44 controls were extracted and purified by TIANamp Macro DNA Kit. Quantitation of plasma DNA was performed by realtime fluores-cence quantitative PCR. ROC curve was used to evaluate the diagnostic value of plasm cell-free DNA for gastric cancer. Results The level of plasma cell-free DNA of patients was significantly higher than that of the controls. In addition,plasma cell-free DNA level was associated with TNM stage（P〈0.05）. Conclusion Quantification of plasma cell-free DNA may be a valuable tool in the diagnostics of gastric cancer.
出处
《中国卫生标准管理》
2016年第1期161-162,共2页
China Health Standard Management
