3种培养基对小球藻生长的影响及在淡水轮虫培育中的应用研究

Effects of three culture media on the growth of Chlorella and its application in culture of freshwater rotifers

运用小球藻培养基SE、BG11和复合肥(CF)进行小球藻的培养,研究不同培养基对小球藻生长的影响,并研究5种不同浓度的CF培养基对小球藻及淡水轮虫培育的影响。结果表明,3种培养基培养小球藻均可取得较好的效果,培养1 d和7 d时,BG11效果最好,CF次之,SE最差,培养16、20和24 d,BG11效果最好,SE次之,CF最差,差异均极显著。5个不同浓度CF培养基中以60 mg/L的培养基,培养小球藻和淡水轮虫效果最好,培养的淡水轮虫密度可达(324.67±37.22)ind/m L,且与其他浓度的培养差异显著。

SE,BG11 and CF（ compound fertilizer） were used to culture Chlorella to study their effects on growth of Chlorella,and the effects of five different concentration of CF were studied on the culture of freshwater rotifers. The results indicated that all the three culture media could obtain good culture in Chlorella. In 1 and 7 days culture,BG11 was the best,CF was followed,SE was the last; in16,20 and 24 days culture,BG11 was the best,SE was followed,CF was the last; the differences were significant. 60 mg / L was the best in the five concentrations of CF in culture of Chlorella and freshwater rotifers,the concentration of rotifers was（ 324. 67 ± 37. 22）ind / m L,and it was significantly different from other groups.