复合PCR方法检测抗草甘膦大豆MON89788

Multiplex PCR for Detection of Transgenic Soybean MON89788

根据抗草甘膦大豆MON89788分子特征,选择大豆内源参照基因(Lectin)、玄参花叶病毒启动子(P-FMV 35S)、豌豆终止子(T-E93')和目的基因(CP4-epsps)4个基因作为复合PCR检测基因,其特异性引物序列参照国家相关标准,对反应条件进行优化并测试其特异性和灵敏度。最终建立了可同时检测除内源基因在外的3种外源基因复合PCR检测体系。

According to the molecular characteristics of glyphosate-resistant soybean MON89788,soybean endogenous reference gene（ Lectin）,figwort mosaic virus promoter（ P-FMV 35S）,peas terminator（ T-E93＇） and the target gene（ CP4-epsps） four gene were selected as detection gene for a multiplex PCR,the sequence of specific primers reference to national standards,and the reaction conditions were optimized and tested for specificity and sensitivity. The multiplex PCR detection system was established including an endogenous gene and three exogenous genes.