儿童节细胞神经母细胞瘤血清特异性蛋白的筛选与鉴定

Detection and identification of serum biomarker for ganglioneuroblastoma in children

目的构建更为完善的儿童节细胞神经母细胞瘤(GNB)的早期诊断血清蛋白质指纹图谱模型。方法入选30例GNB术前患儿及30例正常对照儿童,采集血清标本;以弱阳离子交换磁珠芯片(MB-WCX)处理血清,用表面增强激光解析电离飞行时间质谱(SELDI-TOF-MS)筛选方法检测和分析蛋白m/z峰值,筛选特异性蛋白,通过基质辅助激光解析电离飞行时间质谱(MALDI-TOF/TOF)方法对特异性蛋白进行鉴定。结果 SELDI-TOF-MS质谱筛选GNB术前患儿与正常对照儿童得到m/z峰位于5 920的蛋白标记物,该标记物在GNB术前患儿中高表达(6 180.6±2 328.0),相比正常对照儿童(419.1±493.3),差异有统计学意义(P<0.05);经MALDI-TOF/TOF鉴定,该蛋白标记物为类载脂蛋白C-Ⅲ(Apo C-Ⅲ)。结论 m/z峰位于5 920的蛋白标记物考虑为儿童GNB特异性标记物,其有助于儿童GNB的早期诊断。

Objective To build a more perfect serum protein fingerprint models for early diagnosis of ganglioneuroblastoma（GNB） in children.Methods Thirty children with GNB and 30 normal control children were recruited.Serum samples were collected.Nonspecific serum protein was detected and studied by MB-WCX processing,SELDI-TOF-MS mass spectrometry system and MALDI-TOF/TOF platform.Results Through the SELDL-TOX-MS processing,a peak at 5920 m/z protein markers,and the expression of the markers was high in GNB children（6180.6±2328）,compared with normal control children（419.1±493.3）,the difference was statistically significant（P〈0.05）; MALDI-TOF/TOF platform showed that the protein with a peak at 5920 m/z is identified as Apo C- Ⅲ.Conclusions m/z peak of 5920 protein is suggested as specific biomarker of GNB in children,can provide significant reference for early diagnosis of ganglioneuroblastoma,and prognostic monitoring.