复合微生物菌剂对重茬苹果园土壤细菌群落的影响

Effect of complex microbial agent on the soil bacterial communities in apple replanted orchard

【目的】了解抗重茬复合微生物菌剂对重茬苹果园土壤细菌数量及多样性的影响。【方法】对重茬苹果园土壤进行复合微生物菌剂处理后,采用自动核糖体内转录间隔区序列分析方法(Automated ribosomal intergenic spacer analysis,ARISA)对新茬苹果园、重茬苹果园土壤细菌群落多样性进行分析。【结果】经复合微生物菌剂处理2年后,重茬果园土壤OTUs总数和Shannon-Weiner指数分别为250和4.44,新茬果园OTUs总数和Shannon-Weiner指数分别为234和3.81,经One-Way ANOSIM法分析得到二者差异系数P分别为0.108 3和0.084 3。另外,重茬园和新茬园共享核心OTUs有89个,二者的Jaccard群落相似性系数为0.47,相似程度中等。【结论】复合微生物菌剂具有提高重茬果园土壤微生物多样性,促进重茬苹果园土壤微生物生态系统恢复的作用。

[Objective] To investigate the influence of complex microbial agent on the soil bacterial populations in apple replanted orchard. [Methods] After the treatment with complex microbial agent, soil specimens were sampled from new crop and replanted apple orchard then analyzed by the method of automated ribosomal intergenic spacer analysis to monitor the bacterial community. [Results] Two years after treatment with complex microbial agent, the operational taxonomic units（OTUs） and Shannon-Wiener diversity index of replanted orchard was 250 and 4.44, respectively. In comparison, corresponding values were 234 and 3.81 in new crop orchard. One-Way ANOSIM analysis further showed that the difference coefficient was 0.108 3 and 0.084 3 respectively. In addition, the new crop and replanted orchard shared 89 core OTUs, and had a moderate similarity（corresponding Jaccard community similarity coefficient was 0.47）. [Conclusion] The application of complex microbial agent could improve the soil microbial diversity and benefits to the recovery of the soil microbial ecosystem in replanted orchard.