前康宁胶囊对人前列腺癌细胞22RV1的抗肿瘤作用

Anti-tumor effect of Qiankangning capsule against 22RV1 human prostate adenocarcinoma cell

目的研究前康宁胶囊对人前列腺癌细胞22RV1增殖的影响。方法 1采用MTT法研究前康宁胶囊对22RV1、DU145、PC-3、A549、HCT116和MDA-MB-231等6种人肿瘤细胞株及正常人胚肺细胞WI38体外增殖的抑制作用;2以人前列腺癌细胞22RV1裸鼠异种移植模型评价前康宁胶囊对肿瘤生长的抑制作用;3采用生物素双抗体夹心酶联免疫吸附(ELISA)法检测前康宁胶囊对血清中前列腺特异性抗原(PSA)水平的影响;4采用蛋白印迹法检测前康宁胶囊对CleavedCaspase-3及Cleaved-PARP-1蛋白表达的影响。结果 1前康宁胶囊对各类受试细胞均有不同程度的抑制作用,对22RV1细胞的增殖抑制最为显著;2前康宁胶囊低剂量组、中剂量组、高剂量组,比卡鲁胺低剂量组、高剂量组,前康宁胶囊高剂量+比卡鲁胺低剂量组,及前康宁胶囊高剂量+比卡鲁胺高剂量组,各组的抑瘤率分别为25.21%、39.67%、50.88%、34.28%、53.88%、52.56%及60.24%;3前康宁胶囊低剂量对血清中PSA水平无明显影响,中剂量和高剂量组PSA水平明显降低,抑制率分别为17.83%和33.23%,比卡鲁胺低剂量组和高剂量组的抑制率分别为29.21%和38.48%。前康宁胶囊高剂量+比卡鲁胺低剂量组和前康宁胶囊高剂量+比卡鲁胺高剂量组的抑制率分别为36.93%和41.37%;4蛋白印迹结果显示,前康宁胶囊高剂量组、比卡鲁胺高剂量组及前康宁胶囊高剂量+比卡鲁胺高剂量组活化Caspase-3均显著增加。前康宁胶囊高剂量+比卡鲁胺高剂量组的PARP-1活化水平显著高于空白对照组,前康宁胶囊高剂量组和比卡鲁胺高剂量组均低于空白对照组。结论前康宁胶囊具有一定的抗肿瘤作用,可剂量依赖性地抑制22RV1细胞增殖,对血清PSA水平具有一定抑制作用,其作用机制可能与Caspase-3诱导的细胞凋亡有关。

Objective To study the anti-tumor effect of Qiankangning capsule on 22RV1 human prostate adenocarcinoma cell. Methods ①The anti-proliferative effects of Qiankangning capsule against 6 human carcinoma cells and WI38 cell in vitro were observed by MTT assay. ②The anti-tumor effects of Qiankangning capsule were evaluated with the nude mice bearing 22RV1 xenografts model. ③The effects of Qiankangning capsule on prostate specific antigen （PSA） level were measured by using double-antibody sandwich enzyme-linked immunosorbent assay （ELISA）. ④The effects of Qiankangning capsule on Cleaved-caspase-3 and Cleaved-PARP-1 protein expression were detected by Western blotting. Results ① Qiankangning capsule inhibited 22RV1 cell proliferation mostly. ②The anti-tumor rates of low, middle and high-dose Qiankangning capsule groups, low and high-dose bicalutamide group, high-dose Qiankangning capsule combined with lowdose bicalutamide group and high-dose Qiankangning capsule combined with high-dose bicalutamide group were 25.21%, 39.67%, 50.88%, 34.28%, 53.88%, 52.56% and 60.24% respectively. ③Low-dose Qiankangning capsule had little effect on PSA level, while the middle and high-dose could reduce PSA level with inhibition rate of 17.83% and 33.23%. The PSA inhibition rate of low and high-does bicalutamide were 29.21% and 38.48%. The inhibition rate of high-dose Qiankangning capsule combined with low-dose bicalutamide and high-dose Qiankangning capsule combined with high-dose bicalutamide were 36.93% and 41.37%. ④Western blotting revealed that high-dose Qiankangning capsule, high-dose bicalutamide and high-dose Qiankangning capsule plus high-dose bicalutamide could activate the Caspase-3. The PARP-1 activation level in combination group was higher than that of control group, while those in Qiankangning group and bicalutamide group were lower than control group. Conclusion Qiankangning capsule has anti-tumor effect and can inhibit the proliferation of 22RV1 cell in dose-dependent manner. It can also reduce the level of PSA. The apoptosis induced by active Caspase-3 might be one of the mechanisms.