电针对缺氧缺血性脑病模型大鼠mdm2表达干预研究

Research on Effect of Electroacupuncture on mdm2 Expression in Hypoxic-ischemic Encephalopathy Rat Model

目的:观察电针对新生大鼠缺氧缺血性脑病模型(HIE)mdm2表达的干预机制。方法:7 d龄SD大鼠随机分为假手术组、模型组、假电针组、电针组、拮抗剂组、电针+拮抗剂组,各组又分为1 d、3 d、7 d、21 d四时段,结扎双侧颈总动脉,建造HIE模型。透射电镜观察脑组织超微结构;RT-PCR检测mdm2 m RNA转录水平;Western blot检测mdm2蛋白表达。结果:电镜显示21 d电针组神经元超微结构损伤明显减轻,胞膜恢复完整,染色质尚均匀,偶见肿胀的线粒体。电针组mdm2蛋白表达均下调,与模型组相比,有统计学意义(P<0.05)。结论:电针可明显减轻神经元超微结构退行性变化,其作用机制可能与mdm2表达下调有关,对HIE大鼠发挥神经保护作用。

Objective： To explore the effect of electroacupuncture on the expression of mdm2 following hypoxic-ischemic encephalopathy（ HIE）.Method： The hypoxic-ischemic models were made in new-bore SD rats aged 7 days by colligating bilateral common carotid artery. All rats were randomly divided into sham operation,model,sham acupuncture,electroacupuncture,antagonist and electroacupuncture with antagonist groups which were subdivided into 1 d,3 d,7 d,21 d time-phases. Ultra-structure of neurons was observed by using TEM and real-time PCR was used to determine the level of mdm2 m RNA and the expression of mdm2 was tested with Western Blot.Results： TEM showed that on 21 d,the damnification of neurons had evidently comeback with electroacupuncture treatment. The down-regulation of mdm2 was observed in electroacupuncture group,which was significantly lower than those in model groups（ P〈0. 05）. Conclusion： Electroacupuncture could obviously lighten the damage of ultrastructure of neurons to protect the neurons from HIE by inhibiting the expression of mdm2.