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左、右归丸及其拆方对去卵巢大鼠肝脏抗氧化能力的影响 被引量:2

Effects of Zuogui Pill,Yougui Pill and Demolition Parts on Liver Antioxidant Ability in Ovariectomized Rats
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摘要 目的:探讨左、右归丸及其拆方对去卵巢大鼠肝脏抗氧化能力的影响。方法:手术摘除2月龄SD大鼠双侧卵巢,术后验证造模成功。以左归丸、右归丸、两方共同药、滋阴药、补阳药和阳性对照药补佳乐、蒸馏水给大鼠灌胃。12周后处死,取大鼠肝脏,用冷冻研磨仪将肝脏研磨成10%肝组织匀浆,分别用分光光度法、TBA法测定肝组织匀浆脂质过氧化物(LPO)、丙二醛(MDA)的含量,分别用羟胺法、酶促法、可见光法测肝组织匀浆总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-PX)、过氧化氢酶(CAT)的活性。结果:与模型组比较,左、右归丸及其拆方均能明显降低去卵巢大鼠肝脏LPO、MDA的含量(P<0.05或P<0.01),明显提高肝脏TSOD、GSH-PX、CAT的活性(P<0.01),并且滋阴补肾填精的左归丸组的效果优于右归丸组、共同药组、滋阴药组、补阳药组(P<0.05或P<0.01)。结论:左、右归丸及其拆方均可提高去卵巢大鼠肝脏抗氧化能力,其中滋阴补肾填精的左归丸效果最好。 Objective: To study the influence of Zuogui Pill,Yougui Pill and demolition parts on liver antioxidant ability in ovariectomized rats. Method: By removing bilateral ovaries of the 2-month old SD rats,we made the model successfully. And then the SD rats were given Zuogui Pill,Yougui Pill,mutual drugs of Zuogui Pill and Yougui Pill,nourishing kidney-Yin drugs,tonifying kidney-Yang drugs,positive control drug( Estradiol Valerate,BJL) and distilled water by gavage. After 12 weeks,the SD rats were killed. We took out the rats' liver,grinding the liver into 10% liver tissue homogenate with frozen grinding machine. We determined the contents of homogenate lipid peroxide( LPO) and malondialdehyde( MDA) in the liver tissue respectively by methods of spectrophotometry and thiobarbituric acid and measured the activities of homogenate total superoxide dismutase( T-SOD),glutathione peroxidase( GSH-PX) and catalase( CAT) respectively by hydroxylamine,enzymatic,visible light methods. Results: Compared with the model group,Zuogui Pill,Yougui Pill and their demolition parts all can significantly reduce LPO and MDA in liver of the ovariectomized rats( P〈0. 01 or P〈0.05) and can significantly rise the activities of T-SOD,GSH-PX and CAT( P〈0. 01) and the effect of the nourishing kidney-Yin Zuogui Pill was better than that of Yougui Pill,mutual drugs of Zuogui Pill and Yougui Pill,nourishing kidney-Yin drugs,tonifying kidney-Yang drugs( P〈0. 05 or P〈0. 01). Conclusion: Zuogui Pill,Yougui Pill and their demolition parts all can improve the liver antioxidant ability in ovariectomized rats and the nourishing kidney- Yin Zuogui Pill has a better treatment effect.
出处 《中华中医药学刊》 CAS 北大核心 2016年第2期329-331,共3页 Chinese Archives of Traditional Chinese Medicine
基金 国家自然科学基金项目(81373527)
关键词 左归丸 右归丸 脂质过氧化物 抗氧化酶 Zuogui Pill Yougui Pill lipid peroxide antioxidant enzyme
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