畲药蓬蓬根及根茎的质量标准研究

Quality Standard Study of Traditional She Medicine Roots and Rhizomes of Pengpeng

目的：研究畲药蓬蓬根及根茎的质量标准,为制定合理的质控方法提供科学依据。方法：运用显微鉴别法、理化鉴别法、薄层色谱法、高效液相色谱法分别对蓬蓬根及根茎的显微特征、有效成分进行定性、定量测定;对醇溶性浸出物、水分、总灰分、酸不溶性灰分、重金属及有害元素含量进行控制。结果：蓬蓬根及根茎的薄层色谱、化学反应、显微特征明显;活性成分血根碱和白屈菜红碱的线性范围分别为0.075 5~1.51μg（r=0.999 8）、0.016 5~0.329μg（r=0.999 8）;平均加样回收率分别为97.66%（RSD=1.78%）,98.69%（RSD=1.63%）。结论：研究所得方法简便、准确、重复性好。

Objective： To study the quality standard of Pengpeng＇s roots and rhizomes and to provide the science basis for establishing reasonable methods of quality control. Method： Qualitating and quantitaing tests of the characteristics of the medicine were carried out with the help of microscoptic identification,physical and chemical method of identification,thin-laver chromatography and high performance liquid chromatography. We studied the ethanol soluble extractives,water content,total ash,acid- insoluble substances and the content of heavy metal and harmful elements in samples. Result：Pengpeng＇s roots and rhizomes thin-laver chromatography and microscopive view were obvious. Sanguinarine and chelerythrine were linear in 0.075 5 ~ 1.51 μg and 0.016 5 ~ 0.329 μg respectively. The average recovery rates were 97. 66%（ RSD= 1.78%） and 98.69%（ RSD= 1.63%）. Conclusion： The method is simple,accurate and reproducible and can control the quality of the medicine effectively.