人难治性颞叶癫痫神经细胞凋亡与Caspase3，4的表达

Apoptosis and expression of Caspase 3 and Caspase 4 in neurocytes of refractory human temporal lobe epilepsy

目的观察人难治性颞叶癫痫（TLE）颞叶组织神经细胞凋亡及半胱氨酸天冬氨酸蛋白酶（Caspase）3和Caspase4的表达情况。方法免疫组化S—P染色法检测1993年1月至2008年5月福建医科大学病理学系与福建医科大学附属第一医院神经外科手术切除的30例人难治性颞叶癫痫组（TLE组）与10例脑外伤组（对照组）颞叶组织中神经细胞Caspase3与Caspase4的表达并分析其表达差异，TUNEL法检测两组神经细胞的凋亡情况。结果TLE组颞叶组织神经细胞存在Caspase3和Caspase4的阳性表达且表达明显高于对照组（Caspase3：0．69±0．10比0．26±0．05，t=12．905，P〈0．01；Caspase4：0．62±0．10比0．24±0．05，t=11．880，P〈0．01），TLE组颞叶组织神经细胞凋亡指数（AI）较对照组显著增加（12．6±3．1比2．5±1．9，t=9．664，P〈0．01）。结论人难治性TLE颞叶组织可能存在Caspase3，Caspase4转导的神经细胞凋亡。

Objective To study apoptosis and expression of Caspase 3 and Caspase 4 in temporal lobe neurocytes of refractory human temporal lobe epilepsy （TLE）. Methods The temporal tissue samples were obtained from 30 cases of refractory TLE （TLE group） and 10 cases of brain trauma （contrast group） between January 1993 and May 2008. The surgical specimens were paraffin-embedded samples from Department of Pathology of Fujian Medical University and Department of Neurosurgery, the First Affiliated Hospital of Fujian Medical University. S-P staining of immunohistochemistry was used to detect the expression of Caspase 3 and Caspase 4 in temporal lobe neurocytes of TLE group and contrast group. Their expression was then analyzed. The terminal deoxynucleoitidyl transferase-mediated dUTP nick end labeling （TUNEL） staining was performed to visualize and analyze the neurocytes＇ apoptosis of two groups. Results The expression of Caspase 3 and Caspase 4 in neurocytes of TLE group were both higher than that of contrast group （t = 12. 905, P 〈 0. 01 ; t = 11. 880, P 〈 0. 01 ）. The apoptotic index （AI） of neurocytes in TLE group also increased than that of contrast group （ t = 9. 664, P 〈 0. 01 ）. Conclusion Caspase 3 and Caspase 4 may mediate neurocytes apoptosis in temporal lobe tissue of refractory human TLE.