基于液相色谱-串联质谱法检测多种食品中的9种非食用色素

Determination of 9 illegal pigments in food by LC-MS / MS method

建立了多种食品（葡萄酒、乳饮料、调制乳、海虾,熟鸡蛋,咸鸭蛋,肉制品,辣椒酱,辣椒粉）中的9种非食用色素酸性红1、酸性橙7、酸性黄36、苏丹红Ⅰ、苏丹红Ⅱ、苏丹红Ⅲ、苏丹红Ⅳ、碱性橙31、罗丹明B的液相色谱-串联质谱定量检测方法。分别对提取方式、不同食品的分类预处理方法和质谱条件进行了优化。并选择离子检测进行阳性验证,外标法定量分析了上述食品样品中的色素含量。该方法对于酸性红的最低检出限、线性范围和方法回收率分别为：0.10 mg/kg、0.10~0.50 mg/L和86.5%~95.3%;对于其他8种非食用色素的最低检出限、线性范围和方法回收率分别为：0.010 mg/kg、0.010~0.10 mg/L和86.5%~95.7%。

In this paper,a quantitative method based on liquid chromatography tandem mass spectrometry was established to determine 9 illegal pigments（ Azophloxine,Orange,Metanil yellow,Sudan I,Sudan II,Sudan Ⅲ,Sudan Ⅳ,Basic Orange 31 and Basic rose red） used in various kinds of foods（ wine,milk beverage,blend milk,sea shrimp,hard-boiled eggs,salted duck eggs,meat product,hot pepper sauce,pepper,etc.）. The pre-processing method of different kinds of foods,the extraction method and the mass spectrum conditions of various colorants were optimized. Then the contents of colorants were quantitatively determined by LC-MS / MS using external standard method. The detection limits,linear ranges,and recoveries of this method for Azophloxine,were 0. 10 mg / L,0. 10 ~0. 50 mg / L and 86. 5% ~ 95. 3%,respectively. The detection limits,linear ranges,and recoveries for the other 8pigments were 0. 010 mg / L,0. 010 ~ 0. 10 mg / L and 86. 5 ~ 95. 7%,respectively.