D-900型大孔树脂纯化巴戟天低聚糖的工艺优选

Optimization of Purification Technology for Oligosaccharides in Morindae Officinalis Radix by D-900 Macroporous Resin

目的:优选大孔树脂纯化巴戟天低聚糖的工艺条件,制备高纯度的低聚糖有效部位。方法:以耐斯糖和水溶性低聚糖的吸附-洗脱率为指标,采用单因素试验考察上样液质量浓度、径高比、洗脱剂用量等因素对巴戟天低聚糖大孔树脂纯化工艺的影响。结果:最佳纯化工艺为上样液质量浓度40 g·L-1,树脂柱径高比1∶6,上样量与树脂体积比1∶25,加水12 BV以流速2 BV·h-1洗脱。水洗脱液中低聚糖平均质量分数、吸附-洗脱率、转移率分别为55.70%,94.45%,91.82%。结论:该纯化工艺简单、稳定、成本低,可应用于中试生产。低聚糖有效部位纯度>50%。

Objective： To optimize purification technology of oligosaccharides in Morindae Officinalis Radix by macroporous resin and prepare effective parts of oligosaccharides with high purity. Method： Taking adsorption-desorption rate of nystose and oligosaccharides as indexes, effects of the concentration of sample solution,diameter-height ratio,elution agent and other factors on purification technology of oligosaccharides were investigated by single factor tests. Result： Optimum purification technology was as following： the concentration of sample solution 40 g·L- 1,diameter-height ratio of resin column 1∶ 6,ratio of sample volume-resin 1∶ 25,then eluted with 12 BV of water with flow rate of 2 BV·h- 1,collected eluent. Average content,adsorption-elution rate and transfer rate of oligosaccharides in water eluent were 55. 70%, 94. 95%, 91. 82%, respectively.Conclusion： This optimized purification technology is simple and stable with low cost,purity of oligosaccharides is more than 50%.