HPLC同时测定半枝莲中对香豆酸和肉桂酸的含量

Simultaneous Determination of p-coumaric Acid and Cinnamic Acid in Scutellaria barbata by HPLC

目的：建立半枝莲药材中同时测定对香豆酸和肉桂酸含量的测定方法。方法：采用Eclipse SB-C18色谱柱（4.6 mm×250 mm,5μm）,流动相乙腈-0.2%磷酸水梯度洗脱,流速1 m L·min-1,检测波长310,280 nm,柱温26℃。结果：对香豆酸和肉桂酸分别在0.148~1.776μg（r=0.999 8）,0.062~0.992μg（r=0.999 6）呈良好的线性关系,平均加样回收率分别为99.4%（RSD 1.5%）,99.3%（RSD 1.8%）,供试品溶液在12 h内稳定。通过对8批半枝莲药材的测定,结果发现湖北（批号130501）样品中2种成分含量总体优于其他产地样品。结论：该含量测定方法精密度良好,准确度高,适用于半枝莲药材中对香豆酸和肉桂酸的含量测定。

Objective： To develop a method for simultaneous determining p-coumaric acid and cinnamic acid contents in Scutellaria barbata. Method： Eclipse SB-C18column（ 4. 6 mm × 250 mm,5 μm） was adopted with a mobile phase of acetonitrile-0. 2% phosphoric acid at the flow rate of 1. 0 m L ·min- 1. The detection wavelength was set at 310 nm and 280 nm and the column temperature was 26 ℃. Result： The good linear range was 0. 148-1. 776 μg（ r = 0. 999 8） for p-coumaric acid and 0. 062-0. 992 μg（ r = 0. 999 6） for cinnamic acid.The average recovery rate was 99. 4%（ RSD 1. 5%） and 99. 3%（ RSD 1. 8%） for p-coumaric acid and cinnamic acid respectively. The sample solution was steady within 12 h. Through the determination of the 8 batches of S.barbata,results showed that the contents of these 2 components in Hubei samples（ batch number： 130501） were better than those of other areas. Conclusion： This method is precise and accurate for determination of p-coumaric acid and cinnamic acid contents in S. barbata.