淫羊藿苷对食管癌细胞EC9706增殖与凋亡的影响

Effect of Icariin on Proliferation and Apoptosis of Esophageal Cancer Cells EC9706

目的:探讨淫羊藿苷(icariin,ICA)对食管癌细胞EC9706增殖的影响,明确其作用机制。方法:ICA(40,160,640 nmol·L-1)作用EC9706细胞48 h后,应用CCK8法检测EC9706细胞增殖;应用Hoechest33342染色和荧光显微镜检测细胞凋亡;应用逆转录-聚合酶链反应(RT-PCR)法检测B淋巴细胞瘤-2(Bcl-2),Bcl-2相关X蛋白(Bax)mRNA表达;应用免疫印迹法检测Bax,Bcl-2的蛋白表达。结果:ICA能显著抑制EC9706细胞增殖;ICA作用EC9706细胞出现细胞凋亡形态学改变;免疫印迹结果显示Bax蛋白表达减少,而Bcl-2蛋白表达增多。结论:ICA能显著抑制人食管癌EC9706细胞增殖,其凋亡作用机制可能与上调Bax蛋白,下调Bcl-2蛋白的表达相关。

Objective： To investigate the effect of icariin（ ICA） on the proliferation of esophageal cancer cells EC9706 and identify its mechanism of action. Method： EC9706 cells were stimulated by different concentrations of ICA for 48 h,then CCK8 assay was performed to detect the proliferation of EC9706 cells;Hoechst33342 staining and fluorescence microscope were used to detect cells apoptosis; reverse transcriptionpolymerase charin reaction（ RT-PCR） was used to detect B-cell lymphoma / leukemia2（ Bcl-2） and Bcl-2associated xprotein（ Bax） mRNA expression,Western blot was used to detect Bax and Bcl-2 protein expression.Result： ICA could significantly inhibit proliferation of EC9706 cells. Cell apoptosis morphological changes were found in EC9706 cells after ICA treatment. Western blot results showed Bax protein expression was decreased,while Bcl-2 protein expression was increased. Conclusion： ICA could significantly inhibit the proliferation of human esophageal cancer EC9706 cells,and the mechanism of apoptosis may be associated with up-regulating the expression of Bax protein and down-regulating the expression of Bcl-2 protein.