HPLC法测定木糖母液中的糖组分

Analysis of Sugar Components of Mother Solution of Xylose by HPLC

目的：建立高效液相色谱法测定木糖母液中糖组分的方法。方法：采用AminexHPX一87H柱（7．8mm×300mm），以0．005mol／L硫酸为流动相，流速为0．5mL/min柱温50℃，检测器为示差检测器，进样量20L。结果：以峰面积定量，葡萄糖的线性范围为5～50g（r=0．9999），平均回收率99．8％，RSD=0．80％（n=9）；木糖的线性范围为10～100μg（r=0．9999），平均回收率100．2％，RSD=0．52％（n=9）；阿拉伯糖的线性范围为5-50μg（r=0．9997），平均回收率99．6％，RSD=0．75％（n=9）。结论：该方法简便、结果准确可靠，可用于木糖母液中糖组分的测定。

Objective：To establish HPLC method for analysis of sugar components of mother solution of xyloseo Methods： Aminex HPX-87H column （7.8mm × 300mm） was used. The mobile phase was 0.005mol/L sulfuric acid. The flow rate was0.5mL·min-1. The column temperature was 50 temperature. The detector was refrac -tive index detector （RID）. The injection volume was 20p, L. Results：Quantitative analysis by peak area,the linear of glucose range was 5～50 μg （r=0.9999）. The average recoveries was 99.8% ,RSD=0.80% （n = 9）. The linear of xylose rang was 10-100ixg （r=0.9999）. The average recoveries was 100.2%, RSD=0.52% （n = 9）. The linear of arabinose rang was 5-50 μg （r=0.9997）. The average recoveries was 99.6%, RSD=0.75%（n = 9）. Conclusions：The method is simple, result is reliable and accurate, for the determination the sugar components of xylose mother solution.