摘要
目的探讨地黄低聚糖(RGOs)对过氧化氢-血清饥饿诱导的小型猪脂肪组织来源干细胞(ASCs)Bax、Bcl-2表达的影响。方法过氧化氢(200μM)联合血清饥饿(H2O2/SD,6 h)建立小型猪ASCs凋亡模型。RGOs(0.01 g/L、0.1 g/L、1 g/L、10 g/L)预处理12 h并继续干预6 h。Western-blot法测定细胞Bax、Bcl-2的表达。结果 RGOs在一定浓度范围(0.1 g/L^10 g/L)可以减轻H2O2/SD引起的ASCs损伤,表现在Bax表达下调,Bcl-2表达上调。结论地黄低聚糖对过氧化氢-血清饥饿诱导的脂肪组织来源干细胞的凋亡具有保护作用。
Objective To investigate the effect of Rehmannia glutinosa oligosaccharides (RGOs) on hydrogen peroxide-and serum deprivation - induced apoptosis in adipose tissue-derived stem cells. Methods An apoptosis model in miniswine adipose tissue-derived stem cells (ASCs) was established by hydrogen peroxide-and serum deprivation-induced (H2 O2/SD ,6h)in vitro. RGOs (0.01 g/L,0.1 g/L, 1 g/L, 10g/L) pretreated for 12 hours and continued presence for 6 hours. Expression of Bax and Bcl-2 was investigated by using Western blot analysis. Results RGOs significantly attenuated hydrogen peroxide-and serum deprivation -induced ASCs apoptosis, showing a down -regulated Bax expres sion and an up-regulated Bcl-2 expression with the concentrations between O. lg/L - lOg/L. Conclution Rehmannia glutinosa oligosaccharides can protect adipose tissue-derived stem cells against hydrogen peroxide-and serum deprivationinduced apoptosis.
出处
《中国临床保健杂志》
CAS
2016年第1期69-71,113,共4页
Chinese Journal of Clinical Healthcare
基金
国家高科技研究发展计划(2006AA02A105)
关键词
干细胞移植
地黄
细胞凋亡
过氧化氢
BCL-2相关X蛋白质
模型
动物
Stem cell transplantation
Rehmannia glutinosa
Apoptosis
Hydrogen peroxide
Bcl-2 - Associatedprotein
Models, Animal