Eag1钾离子通道在卵巢癌组织中的表达及意义

Expression and significance ofether à go-go 1 channels in human ovarian cancer

目的检测Etheràgo-go 1(Eag1)钾离子通道在人卵巢癌中的表达,对人卵巢癌细胞株SKOV3的影响及与卵巢癌患者临床特征的关系。方法用实时定量逆转录-聚合酶链反应(qRT-PCR)检测卵巢癌细胞SKOV3中Eag1的表达;用噻唑蓝比色法(MTT)检测Eag1钾离子通道抑制剂阿司咪唑(Astemizole)对SKOV3细胞增殖的影响;用流式细胞仪检测阿司咪唑对对SKOV3细周期的影响;用蛋白印迹法(Western blot)和免疫组织化学的方法检测36例卵巢癌组织中Eag1蛋白的表达并结合临床病例资料进行分析。结果阿司咪唑可明显抑制SKOV3细胞增殖,阻滞细胞周期于G0/G1期。在卵巢癌标本中,Eag1蛋白的阳性表达率明显高于正常卵巢组织。其在不同的临床分期方面差异有统计学意义(P<0.05)。结论 Eag1钾离子通道异常表达于人卵巢癌组织中,并可能成为卵巢癌诊断和治疗的靶点。

Objective To evaluate the expression of Eag1 potassium channel in human ovarian cancer,the effects of Eag1 on human ovarian cancer cell line SKOV3 and the relationship between Eag1 expression and clinicopathological characteristics of ovarian cancer patients. Methods The expression of Eag1 in human ovarian cancer cell line SKOV3 was detected by quantificational reverse transcription polymerase chain reaction（ qRT-PCR）. The effects of astemizole（ a inhibitor of Eag1） on SKOV3 cells proliferation and cycle were measured by MTT and ow cytometry. Then the expressions of Eag1 at protein level were detected by Western blot and immunohistochemical staining in 36 cases of ovarian cancer. The relationship between the index and clinicopathological parameters was analyzed. Results Eag1 was overexpressed in SKOV3 cells. Cell proliferation and cycle can be significantly inhibited by astemizole.Eag1 was expressed higher in the ovarian cancer specimens than in normal ovaries tissues and was associated with clinical stage（ P0.05）. Conclusion The expression of Eag1 potassium channel was aberrant in human ovarian cancer tissues and Eag1 potassium channel may represent a potential target for ovarian cancer diagnosis and treatment.