矾冰纳米乳对大鼠肛瘘术后创面愈合中TGF-β1的影响

Effects of Alum Borneol Nanoemulsion on Expression of TGF-β1 in Rats Wound Healing After Anal Fistula Operation

目的:探讨矾冰纳米乳对大鼠肛瘘术后创面愈合中TGF-β1的影响。方法:建立大鼠肛瘘术后模型,将造模后的30只SD大鼠,随机分为矾冰纳米乳组(A)、湿润烧伤膏组(B)、凡士林组(C),每组各10只。采用相应药物局部涂抹,2次/d,连续用药14天。每天观察创面愈合情况,记录创面愈合时间,于第8、14天计算创面愈合率。分别于术后第8、14天应用免疫组化S-P法检测创面中TGF-β1表达水平。结果:矾冰纳米乳组创面愈合率明显高于润烧伤膏组、凡士林组;矾冰纳米乳组创面愈合时间明显早于湿润烧伤膏组、凡士林组(P<0.05)。术后第第8、14d矾冰纳米乳组TGF-β1的阳性表达率明显高于湿润烧伤膏组、凡士林组,差异均有统计学意义(P<0.05)。结论:矾冰纳米乳可明显提高肛瘘术后创面愈合率,缩短创面愈合时间,其促进肛瘘术后创面愈合的作用机制可能与调节创面愈合中TGF-β1表达有关。

Objective： To investigate the effects of alum borneol nanoemulsion on expression of TGF-β1 in rats wound healing after anal fistula operation. Methods： The author established the model of rats after anal fistula operation, and randomly divided the 30 SD model rats into 3 groups： the alum borneol nanoemulsion group（A）, MEBO group（B）, and vaseline group（C）, each 10 rats. Use the corresponding drugs topical, 2 times/day,and 14 days continuously. Every wound healing was observed, and the wound healing time was recorded in 8,14 days to calculate the rate of wound healing. Respectively after 5, 8, 14 days S-P immunohistochemical method was used to detect the level of TGF-β1 expression on the right side of the wound. Results： The rate of wound healing and the wound healing time of the alum borneol nanoemulsion group was both earlier than MEBO group and vaseline group（P〈0.05）. After 8 and 14 days of treatment, TGF-β1 positive expression rate of alum borneol nanoemulsion group was significantly higher than that in MEBO group and vaseline group（P 〈0.05）. Conclusions： The alum borneol nanoemulsion can significantly improve the rate of wound healing after anal fistula operation, shorten the time of wound healing, action mechanism of its promoting wound healing after anal fistula surgery may be associated with the regulation of wound healing in TGF-β1 expression.